From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2700 [new locus tag: SACOL_RS14125 ]
  • pan locus tag?: SAUPAN006431000
  • symbol: hisB
  • pan gene symbol?: hisB
  • synonym:
  • product: imidazoleglycerol-phosphate dehydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2700 [new locus tag: SACOL_RS14125 ]
  • symbol: hisB
  • product: imidazoleglycerol-phosphate dehydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 2772916..2773494
  • length: 579
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATTTATCAAAAACAACGAAACACAGCTGAAACGCAACTAAATATTTCAATATCTGAT
    GATCAGTCACCATCGCATATTAATACAGGTGTGGGCTTTTTAAATCATATGTTAACCTTG
    TTTACATTTCATAGCGGTCTGTCATTAAACATTGAGGCACAAGGTGATATTGACGTAGAT
    GATCACCACGTAACTGAAGATATCGGCATTGTCATTGGCCAATTGTTACTTGAAATGATT
    AAAGATAAAAAGCATTTCGTTCGTTATGGAACGATGTACATTCCAATGGATGAAACATTA
    GCACGTGTCGTTGTGGATATAAGTGGGCGCCCATACCTATCATTCAATGCATCATTAAGT
    AAAGAAAAAGTTGGTACGTTTGATACGGAGTTAGTAGAAGAATTTTTTAGAGCGGTCGTA
    ATCAATGCAAGATTAACAACGCATATTGATTTAATTCGTGGAGGTAATACACACCATGAA
    ATTGAAGCTATATTCAAAGCGTTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGAT
    GATCAGCGTGTGCCGTCATCGAAAGGTGTGATTGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

⊟Protein[edit | edit source]

Protein Data Bank: 2AE8

⊟General[edit | edit source]

  • locus tag: SACOL2700 [new locus tag: SACOL_RS14125 ]
  • symbol: HisB
  • description: imidazoleglycerol-phosphate dehydratase
  • length: 192
  • theoretical pI: 5.4988
  • theoretical MW: 21456.2
  • GRAVY: -0.0807292

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.19?  ExPASy
    Imidazoleglycerol-phosphate dehydratase D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
  • TIGRFAM:
  • TheSEED  :
    • Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
  • PFAM:
    S5 (CL0329) IGPD; Imidazoleglycerol-phosphate dehydratase (PF00475; HMM-score: 200.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.032907
    • TAT(Tat/SPI): 0.008802
    • LIPO(Sec/SPII): 0.000557
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFHSGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLARVVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHEIEAIFKAFSRALGIALTATDDQRVPSSKGVIE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2700&oldid=78997"