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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2703 [new locus tag: SACOL_RS14140 ]
  • pan locus tag?: SAUPAN006434000
  • symbol: hisG
  • pan gene symbol?: hisG
  • synonym:
  • product: ATP phosphoribosyltransferase catalytic subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2703 [new locus tag: SACOL_RS14140 ]
  • symbol: hisG
  • product: ATP phosphoribosyltransferase catalytic subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2775735..2776349
  • length: 615
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTAAGAATCGCCATAGCCAAAGGACGTCTAATGGATAGTTTAATTAACTATTTAGAT
    GTAATTGAATATACGACATTATCAGAAACATTAAAAAATAGAGAACGCCAATTATTATTA
    AGTGTAGATAATATTGAATGCATTTTAGTAAAAGGAAGTGACGTGCCAATCTATGTGGAA
    CAAGGAATGGCAGACATAGGCATTGTTGGTAGCGACATATTAGATGAGCGCCAATATAAT
    GTTAATAATTTGTTGAATATGCCTTTTGGAGCATGTCATTTTGCGGTTGCAGCGAAACCT
    GAAACGACCAATTATCGTAAAATCGCAACGAGTTATGTTCATACTGCTGAAACATATTTT
    AAATCAAAAGGTATTGATGTCGAATTGATTAAATTGAATGGCTCTGTTGAATTGGCCTGT
    GTTGTAGATATGGTAGACGGAATTGTCGACATCGTTCAAACAGGTACTACGCTAAAAGCG
    AACGGACTGGTTGAAAAGCAACATATTAGTGATATCAATGCAAGATTAATAACTAATAAA
    GCAGCTTATTTTAAAAAATCACAATTAATAGAGCAATTTATTCGCTCTTTGGAGGTGTCT
    ATTGCCAATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    615

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2703 [new locus tag: SACOL_RS14140 ]
  • symbol: HisG
  • description: ATP phosphoribosyltransferase catalytic subunit
  • length: 204
  • theoretical pI: 5.32275
  • theoretical MW: 22643
  • GRAVY: 0.0897059

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.17?  ExPASy
    ATP phosphoribosyltransferase 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase (TIGR00070; EC 2.4.2.17; HMM-score: 181.6)
  • TheSEED  :
    • ATP phosphoribosyltransferase (EC 2.4.2.17) => HisGs
  • PFAM:
    PBP (CL0177) HisG; ATP phosphoribosyltransferase (PF01634; HMM-score: 158.7)
    and 1 more
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00278
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.000208
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDNIECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPETTNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKANGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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