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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2701 [new locus tag: SACOL_RS14130 ]
  • pan locus tag?: SAUPAN006432000
  • symbol: SACOL2701
  • pan gene symbol?: —
  • synonym:
  • product: histidinol-phosphate aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2701 [new locus tag: SACOL_RS14130 ]
  • symbol: SACOL2701
  • product: histidinol-phosphate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2773463..2774476
  • length: 1014
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTTATATTGATAAAAATGAAAGTCCAGTTACGCCGTTGGATGAAAAAACAATGACG
    TCTATTATTAGTGCAACGCCATATAATTTATATCCTGATGCAGCATATGAACAATTCAAG
    GAAGCTTATGCTAAGTTTTACGGATTATCGCCTGAACAAATTATTGCAGGAAATGGATCT
    GATGAATTGATTCAAAAGTTAATGCTGATCATGCCAGAAGGTCCGGCATTAACGCTAAAT
    CCTGATTTTTTTATGTATCAAGCATATGCGGCACAAGTAAATCGTGAAATTGCATTTGTA
    GATGCAGGATCAGATTTAACGTTTGATTTGGAAACCATTTTAACGAAAATCGATGAAGTA
    CAACCATCATTTTTTATTATGAGTAATCCACATAACCCTTCAGGCAAGCAATTTGATACG
    GCATTTTTAACAGCTATTGCAGATAAGATGAAAGCATTAAACGGATACTTTGTCATTGAT
    GAAGCATATTTAGATTATGGTACGGCATATGACGTGGAACTGGCACCACACATCTTAAGA
    ATGCGTACATTATCAAAGGCGTTTGGAATTGCCGGCTTAAGATTAGGTGTCTTAATTAGT
    ACTGCTGGAACGATAAAGCATATTCAAAAAATAGAACATCCATATCCATTAAATGTATTT
    ACGCTAAATATTGCGACTTATATTTTTAGACATAGAGAAGAGACAAGACAATTTTTAACG
    ATGCAACGACAGTTAGCTGAGCAGTTAAAACAAATATTTGATACACATGTTGCAGATAAA
    ATGTCAGTGTTCCCATCAAATGCTAATTTTGTACTTACTAAAGGCTCAGCAGCGCAACAA
    TTAGGACAATACGTATATGAACAAGGATTTAAACCTCGCTTTTATGATGAGCCGGTGATG
    AAGGGCTATGTAAGATACTCAATTGCAACAGCATCACAGTTAAAGCAATTAGAAGAAATT
    GTTAAAGAATGGAGTGCAAAATATGATTTATCAAAAACAACGAAACACAGCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2701 [new locus tag: SACOL_RS14130 ]
  • symbol: SACOL2701
  • description: histidinol-phosphate aminotransferase
  • length: 337
  • theoretical pI: 5.65809
  • theoretical MW: 38240.5
  • GRAVY: -0.201187

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 198.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 163.9)
    and 8 more
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 46)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 31.9)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 27.7)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 26.7)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 19.9)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 18.3)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 17.9)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 15)
  • TheSEED  :
    • Similar to histidinol-phosphate aminotransferase
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Histidinol-phosphate aminotransferase (EC 2.6.1.9)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Histidinol-phosphate aminotransferase (EC 2.6.1.9)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 126.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0182
    • TAT(Tat/SPI): 0.000757
    • LIPO(Sec/SPII): 0.00112
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYAKFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDAGSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVIDEAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLNVFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGSAAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTTKHS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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