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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2697 [new locus tag: SACOL_RS14110 ]
  • pan locus tag?: SAUPAN006428000
  • symbol: hisF
  • pan gene symbol?: hisF
  • synonym:
  • product: imidazole glycerol phosphate synthase subunit HisF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2697 [new locus tag: SACOL_RS14110 ]
  • symbol: hisF
  • product: imidazole glycerol phosphate synthase subunit HisF
  • replicon: chromosome
  • strand: -
  • coordinates: 2770889..2771647
  • length: 759
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATTAAAAAACGTATCATTCCATGTTTAGATGTCAAAGATGGTCGTGTCGTTAAAGGG
    ATTCAATTTAAAGGATTAAGGGATATTGGGAATCCTGTTGATTTAGCAATGTATTACAAT
    GAAGCGGGTGCTGATGAATTAGTATTTTTAGACATCTCTAAGACGGAAGAGGGTCATAGC
    TTAATGCTAGAAGTGATTGAACAGACAGCGTCACGCTTGTTTATCCCTCTTACTGTAGGG
    GGTGGGATTCAAAGTCTCGATGATATTACCCAATTGCTAAATCATGGTGCAGATAAAGTA
    TCATTAAATTCAAGTGCTTTAAAAAATCCACAGCTCATTAAACAAGCGAGTGATAAATTC
    GGTAGACAATGCATCTGCATAGCAATTGATAGCTATTATGATCCTGAAAGAAAAGCACAT
    TATTGTTGTACGACTGGTGGTAAAAAAATGACAAATATTAAAGTATATGACTGGGTACAG
    CAAGTAGAACAGTTAGGTGCAGGTGAGCTCCTCGTTACAAGTATGGGACATGATGGTATG
    AAACAAGGCTTTGATATTGAACACCTAGCAAATATTAAGTCTCTTGTAAATATTCCAATC
    ATTGCTTCTGGTGGTGGTGGCAATGCACAACACTTTGTAGAATTATTTGATCAGACGGAT
    GTTTCTGCAGGTTTAGCTGCAAGTATATTACATGATCGAGAAACGACGGTTCAATCTATT
    AAAGAAGTGATACGGCAAGGGGGTATAGCAGTAAGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    759

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2697 [new locus tag: SACOL_RS14110 ]
  • symbol: HisF
  • description: imidazole glycerol phosphate synthase subunit HisF
  • length: 252
  • theoretical pI: 6.15747
  • theoretical MW: 27498.4
  • GRAVY: -0.097619

⊟Function[edit | edit source]

  • reaction:
    5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O?
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 334.3)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 271.3)
    and 12 more
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 118.7)
    bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 74.2)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 34.6)
    Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 33.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 24.3)
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 18.7)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 16.2)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 14.3)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 14.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 13.6)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 12.9)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 12.3)
  • TheSEED  :
    • Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)
  • PFAM:
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 266.3)
    and 7 more
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 29.3)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 26.6)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 21.7)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 20)
    PK_TIM (CL0151) PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 13.8)
    TIM_barrel (CL0036) ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 13)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003209
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.000378
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGADELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSLNSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQQVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQTDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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