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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03012
  • pan locus tag?: SAUPAN006432000
  • symbol: SAOUHSC_03012
  • pan gene symbol?: —
  • synonym:
  • product: histidinol-phosphate aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03012
  • symbol: SAOUHSC_03012
  • product: histidinol-phosphate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2785375..2786388
  • length: 1014
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTTATATTGATAAAAATGAAAGTCCAGTTACGCCGTTGGATGAAAAAACAATGACG
    TCTATTATTAGTGCAACGCCATATAATTTATATCCTGATGCAGCATATGAACAATTCAAG
    GAAGCTTATGCTAAGTTTTACGGATTATCGCCTGAACAAATTATTGCAGGAAATGGATCT
    GATGAATTGATTCAAAAGTTAATGCTGATCATGCCAGAAGGTCCGGCATTAACGCTAAAT
    CCTGATTTTTTTATGTATCAAGCATATGCGGCACAAGTAAATCGTGAAATTGCATTTGTA
    GATGCAGGATCAGATTTAACGTTTGATTTGGAAACCATTTTAACGAAAATCGATGAAGTA
    CAACCATCATTTTTTATTATGAGTAATCCACATAACCCTTCAGGCAAGCAATTTGATACG
    GCATTTTTAACAGCTATTGCAGATAAGATGAAAGCATTAAACGGATACTTTGTCATTGAT
    GAAGCATATTTAGATTATGGTACGGCATATGACGTGGAACTGGCACCACACATCTTAAGA
    ATGCGTACATTATCAAAGGCGTTTGGAATTGCCGGCTTAAGATTAGGTGTCTTAATTAGT
    ACTGCTGGAACGATAAAGCATATTCAAAAAATAGAACATCCATATCCATTAAATGTATTT
    ACGCTAAATATTGCGACTTATATTTTTAGACATAGAGAAGAGACAAGACAATTTTTAACG
    ATGCAACGACAGTTAGCTGAGCAGTTAAAACAAATATTTGATACACATGTTGCAGATAAA
    ATGTCAGTGTTCCCATCAAATGCTAATTTTGTACTTACTAAAGGCTCAGCAGCGCAACAA
    TTAGGACAATACGTATATGAACAAGGATTTAAACCTCGCTTTTATGATGAGCCGGTGATG
    AAGGGCTATGTAAGATACTCAATTGCAACAGCATCACAGTTAAAGCAATTAGAAGAAATT
    GTTAAAGAATGGAGTGCAAAATATGATTTATCAAAAACAACGAAACACAGCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_03012
  • symbol: SAOUHSC_03012
  • description: histidinol-phosphate aminotransferase
  • length: 337
  • theoretical pI: 5.65809
  • theoretical MW: 38240.5
  • GRAVY: -0.201187

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.9?  ExPASy
    Histidinol-phosphate transaminase L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 198.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 163.9)
    and 8 more
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 46)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 31.9)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 27.7)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 26.7)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 19.9)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 18.3)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 17.9)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 15)
  • TheSEED  :
    • Similar to histidinol-phosphate aminotransferase
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Histidinol-phosphate aminotransferase (EC 2.6.1.9)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Histidinol-phosphate aminotransferase (EC 2.6.1.9)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 126.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0182
    • TAT(Tat/SPI): 0.000757
    • LIPO(Sec/SPII): 0.00112
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYAKFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDAGSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVIDEAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLNVFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGSAAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTTKHS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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