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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03014
  • pan locus tag?: SAUPAN006434000
  • symbol: hisG
  • pan gene symbol?: hisG
  • synonym:
  • product: ATP phosphoribosyltransferase catalytic subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03014
  • symbol: hisG
  • product: ATP phosphoribosyltransferase catalytic subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2787647..2788261
  • length: 615
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTAAGAATCGCCATAGCCAAAGGACGTCTAATGGATAGTTTAATTAACTATTTAGAT
    GTAATTGAATATACGACATTATCAGAAACATTAAAAAATAGAGAACGCCAATTATTATTA
    AGTGTAGATAATATTGAATGCATTTTAGTAAAAGGAAGTGACGTGCCAATCTATGTGGAA
    CAAGGAATGGCAGACATAGGCATTGTTGGTAGCGACATATTAGATGAGCGCCAATATAAT
    GTTAATAATTTGTTGAATATGCCTTTTGGAGCATGTCATTTTGCGGTTGCAGCGAAACCT
    GAAACGACCAATTATCGTAAAATCGCAACGAGTTATGTTCATACTGCTGAAACATATTTT
    AAATCAAAAGGTATTGATGTCGAATTGATTAAATTGAATGGCTCTGTTGAATTGGCCTGT
    GTTGTAGATATGGTAGACGGAATTGTCGACATCGTTCAAACAGGTACTACGCTAAAAGCG
    AACGGACTGGTTGAAAAGCAACATATTAGTGATATCAATGCAAGATTAATAACTAATAAA
    GCAGCTTATTTTAAAAAATCACAATTAATAGAGCAATTTATTCGCTCTTTGGAGGTGTCT
    ATTGCCAATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    615

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_03014
  • symbol: HisG
  • description: ATP phosphoribosyltransferase catalytic subunit
  • length: 204
  • theoretical pI: 5.32275
  • theoretical MW: 22643
  • GRAVY: 0.0897059

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.17?  ExPASy
    ATP phosphoribosyltransferase 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase (TIGR00070; EC 2.4.2.17; HMM-score: 181.6)
  • TheSEED  :
    • ATP phosphoribosyltransferase (EC 2.4.2.17) => HisGs
  • PFAM:
    PBP (CL0177) HisG; ATP phosphoribosyltransferase (PF01634; HMM-score: 158.7)
    and 1 more
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 15.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00278
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.000208
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDNIECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPETTNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKANGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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