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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00270 [old locus tag: SA0023 ]
  • pan locus tag?: SAUPAN000040000
  • symbol: SA_RS00270
  • pan gene symbol?: orfX
  • synonym: rlmH
  • product: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00270 [old locus tag: SA0023 ]
  • symbol: SA_RS00270
  • product: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
  • replicon: chromosome
  • strand: +
  • coordinates: 33692..34171
  • length: 480
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (33692..34171) NCBI
  • BioCyc: SA_RS00270 BioCyc
  • MicrobesOnline: see SA0023

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAATCACCATTTTAGCTGTAGGGAAACTAAAAGAGAAATATTGGAAGCAAGCCATA
    GCAGAATATGAAAAACGTTTAGGCCCATACACCAAGATAGACATCATAGAAGTTCCAGAC
    GAAAAAGCACCAGAAAATATGAGCGACAAAGAAATTGAGCAAGTAAAAGAAAAAGAAGGC
    CAACGAATACTAGCCAAAATTAAACCACAATCCACAGTCATTACATTAGAAATACAAGGA
    AAGATGCTATCTTCCGAAGGATTGGCCCAAGAATTGAACCAACGCATGACCCAAGGGCAA
    AGCGACTTTGTATTCGTCATTGGCGGATCAAACGGCCTGCACAAGGACGTCTTACAACGC
    AGTAACTACGCACTATCATTCAGCAAAATGACATTCCCACATCAAATGATGCGGGTTGTG
    TTAATTGAGCAAGTGTATAGAGCATTTAAGATTATGCGTGGAGAAGCATATCATAAATGA
    60
    120
    180
    240
    300
    360
    420
    480

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS00270 [old locus tag: SA0023 ]
  • symbol: SA_RS00270
  • description: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
  • length: 159
  • theoretical pI: 9.85885
  • theoretical MW: 18306.2
  • GRAVY: -0.461635

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.177?  ExPASy
    23S rRNA (pseudouridine1915-N3)-methyltransferase S-adenosyl-L-methionine + pseudouridine1915 in 23S rRNA = S-adenosyl-L-homocysteine + N3-methylpseudouridine1915 in 23S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification rRNA large subunit m3Psi methyltransferase RlmH (TIGR00246; EC 2.1.1.177; HMM-score: 186.6)
  • TheSEED: see SA0023
  • PFAM:
    SPOUT (CL0098) SPOUT_MTase; Predicted SPOUT methyltransferase (PF02590; HMM-score: 206.9)
    and 5 more
    Methyltrn_RNA_4; SAM-dependent RNA methyltransferase (PF09936; HMM-score: 16.3)
    no clan defined ExbD; Biopolymer transport protein ExbD/TolR (PF02472; HMM-score: 13.2)
    GT-A (CL0110) Glyco_tranf_2_3; Glycosyltransferase like family 2 (PF13641; HMM-score: 12.9)
    no clan defined SCAB-IgPH; Fused Ig-PH domain of plant-specific actin-binding protein (PF16709; HMM-score: 12.5)
    PDDEXK (CL0236) Vsr; DNA mismatch endonuclease Vsr (PF03852; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008899
    • TAT(Tat/SPI): 0.00034
    • LIPO(Sec/SPII): 0.002259
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAPENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSDFVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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