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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS00615 [old locus tag: SA0091 ]
  • pan locus tag?: SAUPAN000858000
  • symbol: SA_RS00615
  • pan gene symbol?: plc
  • synonym:
  • product: 1-phosphatidylinositol phosphodiesterase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS00615 [old locus tag: SA0091 ]
  • symbol: SA_RS00615
  • product: 1-phosphatidylinositol phosphodiesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 102646..103632
  • length: 987
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (102646..103632) NCBI
  • BioCyc: SA_RS00615 BioCyc
  • MicrobesOnline: see SA0091

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAGTGTATTAAGACTTTGTTTTTAAGTATCATTTTAGTAGTGATGGGTGGTTGG
    TATCATTCAGCACATGCGTCAGATTCGTTGAGTAAAAGTCCAGAAAATTGGATGAGTAAA
    CTTGATGAAAGCAAACATTTAACTGAGATTAATATGCCAGGCTCACATGATAGTGGCTCA
    TTCACTTTAACTGATCCGGTAAAATCGGTTTGGGCAAAGACTCAAGGCAAAGATTACCTT
    ACTCAAATGAAGTCGGGAGTCAGGTTTTTTGATATTAGAGGTAGAGCAAGTGCTGATAAT
    ATGATTTCAGTTCATCACGGCATGGTTTATTTGCATCATGAACTAGGAAAATTTTTAGAT
    GATGCTAAATATTACTTGAGTGCTTATCCAAACGAAACAATTGTGATGTCTATGAAAAAG
    GACTACGATAGCGATTCTAAAGTTACGAAGACATTTGAAGAGATTTTTAGAGAATATTAT
    TATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTA
    AAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACATACATAAAGAGT
    GGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAA
    ATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAA
    AAAGTTGAAGCTGTTAAAAATTTATTGGCTAAAGCTAAAACGGATAGTAACAAAGACAAT
    GTATATGTGAATTTCTTGAGTGTAGCGTCTGGAGGTAGCGCATTTAATAGTACTTATAAC
    TATGCATCACATATAAATCCTGAAATTGCAAAAACGATTAAAGAAAACGGGAAAGCTAGA
    ACTGGTTGGCTGATTGTTGACTATGCAGGATATCCGTGGCCTGGATATGATGATATCGTA
    AGTGAAATTATAGATAGTAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS00615 [old locus tag: SA0091 ]
  • symbol: SA_RS00615
  • description: 1-phosphatidylinositol phosphodiesterase
  • length: 328
  • theoretical pI: 8.2827
  • theoretical MW: 37087.5
  • GRAVY: -0.56311

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0091
  • PFAM:
    PLC (CL0384) PI-PLC-X; Phosphatidylinositol-specific phospholipase C, X domain (PF00388; HMM-score: 64.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.992705
    • TAT(Tat/SPI): 0.001038
    • LIPO(Sec/SPII): 0.003005
    • Cleavage Site: CS pos: 26-27. AHA-SD. Pr: 0.9796
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKCIKTLFLSIILVVMGGWYHSAHASDSLSKSPENWMSKLDESKHLTEINMPGSHDSGSFTLTDPVKSVWAKTQGKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFNSTYNYASHINPEIAKTIKENGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]