PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00815 [old locus tag: SA0130 ]
• pan locus tag^?: SAUPAN000946000
• symbol: SA_RS00815
• pan gene symbol^?: —
• synonym:
• product: GntR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00815 [old locus tag: SA0130 ]
• symbol: SA_RS00815
• product: GntR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 149935..150690
• length: 756
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (149935..150690) NCBI
• BioCyc: SA_RS00815 BioCyc
• MicrobesOnline: see SA0130

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGGAGGTTGTCTACGTGACGCAAACACAACCAAAATTTTTAACGATTTATAATACGTTA
  TATAAAGAAATTCAAATAGGAAAATATCCGAGTGGCCATGCATTACCTACTGAAAAGGAA
  CTTTGTGCGCGCTTTGATGTCAGTCGTATGACTTTGCGCCAAGCTATAAAATTGTTAGCC
  GAAGATGGCATCGTAGAGAGTACAAGAGGGAAGGGACACTTTGTTATTCCACAAACAAAT
  GCCCAACACGCTTCAAGTATGGATCAATTTAAGCATCCACTTGATCAAATATTGCTAGCT
  CGCGTATCTATGTCATCAATTAATTATCGTGTTGATTTAGAAAGTGAATATACAAATCAT
  TTGTTTGCAAACCATCCTTCTGCCGTCATTGCAATGGAACGCTATTATCAAAAAAAGGAC
  AATCATTCTAAGCAAGCTGATGCATTTTGCTTTACCTTTATACCGTTAAATGTCATTGAC
  ACATTTAAAGTGAATACACAAAACGAAGACGACATGAAGACATTCGTTGAGCAAACGATT
  TATAGCAATGCATATCAATCAGATTTAAAAATGTCTATTACCAAAGCGCCCCGCTTCAAA
  AATCATAGTCACGTGTTTGATGGTGATACACATTGTTGGCTGATTATTGAAACACTGTAC
  GCACAAACACCTTATCCAATTATGATTAATAAGTGGTATATTCCACAGGAAATATCTGAA
  TTAACACTGACACGTATAAGACAATCGGATTATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  756
  

⊟Protein

⊟General

• locus tag: SA_RS00815 [old locus tag: SA0130 ]
• symbol: SA_RS00815
• description: GntR family transcriptional regulator
• length: 251
• theoretical pI: 7.06841
• theoretical MW: 29190
• GRAVY: -0.4251

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 68.1)
  Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 55.9)
  Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 55.9)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 55)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 55)
  and 7 more
  Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 42)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
  Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 35.8)
  phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 24.7)
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 14.9)
  Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 12.7)
• TheSEED: see SA0130
• PFAM:
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 62.7)
  and 12 more
  HTH_11; HTH domain (PF08279; HMM-score: 29.2)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 21.6)
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 20.8)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 20)
  HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 17)
  MarR_2; MarR family (PF12802; HMM-score: 16.5)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.9)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.8)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13.7)
  no clan defined PASTA; PASTA domain (PF03793; HMM-score: 13.4)
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13.2)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00499
  • TAT(Tat/SPI): 0.000149
  • LIPO(Sec/SPII): 0.000385
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SA0130
• RefSeq: WP_001795345 NCBI
• UniProt: see SA0130

⊟Protein sequence

• MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCARFDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARVSMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVIDTFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPRFKNHSHVFDGDTHCWLIIETLYAQTPYPIMINKWYIPQEISELTLTRIRQSDY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications