PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02205 [old locus tag: SA0385 ]
• pan locus tag^?: SAUPAN002107000
• symbol: SA_RS02205
• pan gene symbol^?: ssl4
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02205 [old locus tag: SA0385 ]
• symbol: SA_RS02205
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 444488..445366
• length: 879
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (444488..445366) NCBI
• BioCyc: SA_RS02205 BioCyc
• MicrobesOnline: see SA0385

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGAAAATAACAACAATTGCTAAAACAAGTTTAGCACTAGGCCTTTTAACAACGGGTGTA
  ATCACAACGACAACGCAAGAAGCAAACGCGACAACACCATCTTCCACTAAAGTGGAAGCA
  CCACAATCAACACCGCCCTCAACTAAAGTAGAAGCACCGCAATCAAAACCAAACGCGACA
  ACACCGCCTTCAACTAAAGTGGAAACACCGCAACAAACACCGAACGCGACAACACCATCT
  TCAACTAAAGTGGAAACACCACAATCGCCAACCACAAAACAAGTACCAACAGAAATAAAT
  CCTAAATTTAAAGATTTAAGAGCGTATTATACGAAACCAAGTTTAGAATTTAAAAATGAG
  ATTGGTATTATTTTAAAAAAATGGACGACAATAAGATTTATGAATATTGTTCCAGATTAT
  TTCATATATAAAATAGCTTTAGTTGGAAAAGACGATAAAAAATATGATGAAGGAGTACAT
  AGGAATGTCGATGTATTTGTCGTTTTAGAAGAAAAAAATAAATATGGAGTGGAAAGATAC
  TCGGTCGGTGGTATCACAAAGAGTAATAGTAAAAAAGTTGATCACAAAGCAGGAGTAAGA
  ATTACTAAAGAAGATAATAAAGGTACAATCTCTCATGATGTTTCAGAATTCAAGATTACT
  AAAGAGCAGATTTCCTTGAAAGAACTTGATTTTAAATTGAGAAAACAACTTATTGAAAAT
  CATAATCTGTACGGTAACGTTGGTTCAGGTAAAATTGTTATTAACATGAAAAACGGTGGA
  AAGTACACGTTTGAATTACACAAAAAATTACAAGAAAATCGCATGGCAGACGTCATAGAT
  GGCACTAATATTGATAACATTGAAGTGAATATAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  879
  

⊟Protein

⊟General

• locus tag: SA_RS02205 [old locus tag: SA0385 ]
• symbol: SA_RS02205
• description: hypothetical protein
• length: 292
• theoretical pI: 10.0612
• theoretical MW: 32540.9
• GRAVY: -0.635616

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair UV excision repair protein Rad23 (TIGR00601; HMM-score: 3.3)
• TheSEED: see SA0385
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 105)
  and 8 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 32.4)
  no clan defined PRCC; Mitotic checkpoint regulator, MAD2B-interacting (PF10253; HMM-score: 17.2)
  Rota_NS26; Rotavirus NS26 (PF01525; HMM-score: 14.4)
  GP88; Gene 88 protein (PF17338; HMM-score: 12.3)
  Toxin_10; Insecticidal Crystal Toxin, P42 (PF05431; HMM-score: 12.1)
  Nucleo_P87; Nucleopolyhedrovirus capsid protein P87 (PF07267; HMM-score: 9.8)
  FAP; Fibronectin-attachment protein (FAP) (PF07174; HMM-score: 6.7)
  Ndc1_Nup; Nucleoporin protein Ndc1-Nup (PF09531; HMM-score: 5.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.956052
  • TAT(Tat/SPI): 0.014616
  • LIPO(Sec/SPII): 0.022561
  • Cleavage Site: CS pos: 30-31. ANA-TT. Pr: 0.8010
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446628311 NCBI
• RefSeq: WP_000705657 NCBI
• UniProt: see SA0385

⊟Protein sequence

• MKITTIAKTSLALGLLTTGVITTTTQEANATTPSSTKVEAPQSTPPSTKVEAPQSKPNATTPPSTKVETPQQTPNATTPSSTKVETPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNIVPDYFIYKIALVGKDDKKYDEGVHRNVDVFVVLEEKNKYGVERYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIENHNLYGNVGSGKIVINMKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNIK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)