PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02350 [old locus tag: SA0411 ]
• pan locus tag^?: SAUPAN002163000
• symbol: SA_RS02350
• pan gene symbol^?: mpsA
• synonym:
• product: NADH dehydrogenase subunit 5

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02350 [old locus tag: SA0411 ]
• symbol: SA_RS02350
• product: NADH dehydrogenase subunit 5
• replicon: chromosome
• strand: +
• coordinates: 470927..472411
• length: 1485
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (470927..472411) NCBI
• BioCyc: SA_RS02350 BioCyc
• MicrobesOnline: see SA0411

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1441
  
  GTGTTAAGTTTTCAATTGCTATTTTCACTGTTTGTTATTGCGCTTATCATTGCATTGATA
  AGTGGCTTGTTGTTTTTAGCACCAGTTATGCCAATGAGATATATTAAATTACATTTATAC
  ATACTAGTCATGCCAGTATTATTTGCAGTCATTGGCTTTTTCGGTATTCATGGTCAACAT
  GTCTTAGGTCCATTTAAAATAGATCGTTTATCTTGGTTATTAGCTGGCTTTGTAATGGCG
  CTTGGTTTTATTATTCAAAAGTTTTCAATGCGATATTTACTAGGTGATCATCATTATAGA
  CATTACTTTCCATTGTTCACTGCGATTACGTCGTTTGCATCTTTAGCATGGATGTCTGAA
  GACTTAAGACTGATGGCACTCTGCTGGGGTATGACATTATTATGTTTAACACTGCTGATG
  AACGTTAATCGTTTTTGGAAAGTGCCACGTGAGTCTGCGAAATTATCAAGCATGACATTT
  TTATGTGGTTGGCTTGCATTCGTTGGAGCAATTGTAACTATTTATATTGCGACTGGTGAG
  TGGCGGGTACCACAACATATAGTACATCCGACATGGTCATTGTTGACGAATGTACTACTT
  GTATTAGCTGTCATGATACCGGCAGCACAATTTCCTTTTCATCGATGGTTGATTGAATCT
  GTAACGGCACCAACGCCAGTATCGGCAATTATGCATGCAGGAATTGTGAATGCAGGTGGT
  GTTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTT
  ATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTGGTTCAAGTTGACTAC
  AAAAGACAGTTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCA
  TTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACA
  TTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCT
  AAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTA
  TTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGCATGG
  TCATTACTTGTATCTTGGAATCAGATGGTAGCCTTTAGTAAAGGGCGCATGGCACGTCTG
  GTTGGCATGATTTTGATTGCAATTGTGACATTTATCTACGTCATCACACACAATTATTTT
  TACGATGTATTACAAAATATAACAACACATGCGACAACACCGCCTACAGTGAGTGTCATC
  ATTAGTGTTGTCATTTTAATCTTTGGCAGTTTATTAAGTATTTGGGTGGCGCGTCATCGA
  TACTCTAAGGGTTTTGCGGTATTGTACGTGTGGTTAGTTAATCTAGGTGAAGCACGCTCG
  AAAGCGATAGAAAGTCATCCGAATTATTTGAAGAAGTATTTATAG
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  1485
  

⊟Protein

⊟General

• locus tag: SA_RS02350 [old locus tag: SA0411 ]
• symbol: SA_RS02350
• description: NADH dehydrogenase subunit 5
• length: 494
• theoretical pI: 10.2961
• theoretical MW: 55318.2
• GRAVY: 0.901417

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain L (TIGR01974; EC 1.6.-.-; HMM-score: 134.7)
  and 4 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00940; HMM-score: 88.7)
  NAD(P)H dehydrogenase, subunit NdhF3 family (TIGR01960; HMM-score: 59.3)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain M (TIGR01972; HMM-score: 50.8)
  Metabolism Energy metabolism Electron transport proton-translocating NADH-quinone oxidoreductase, chain N (TIGR01770; HMM-score: 23)
• TheSEED: see SA0411
• PFAM:
  ComplexI-N (CL0425) Proton_antipo_M; Proton-conducting membrane transporter (PF00361; HMM-score: 175.4)
  and 1 more
  no clan defined Proton_antipo_N; NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus (PF00662; HMM-score: 22.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004434
  • TAT(Tat/SPI): 0.00094
  • LIPO(Sec/SPII): 0.018181
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 446871039 NCBI
• RefSeq: WP_000948295 NCBI
• UniProt: see SA0411

⊟Protein sequence

• MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVMPVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHYFPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTFLCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLIESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLVQVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNIPKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAFSKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGSLLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications