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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02590 [old locus tag: SA0449 ]
  • pan locus tag?: SAUPAN002228000
  • symbol: SA_RS02590
  • pan gene symbol?: —
  • synonym:
  • product: TatD family deoxyribonuclease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02590 [old locus tag: SA0449 ]
  • symbol: SA_RS02590
  • product: TatD family deoxyribonuclease
  • replicon: chromosome
  • strand: +
  • coordinates: 521192..521965
  • length: 774
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (521192..521965) NCBI
  • BioCyc: SA_RS02590 BioCyc
  • MicrobesOnline: see SA0449

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTAATCGATACACATGTCCATTTAAATGATGAGCAATACGATGATGATTTGAGTGAA
    GTGATTACACGTGCTAGAGAAGCAGGTGTTGATCGTATGTTTGTAGTTGGTTTTAACAAA
    TCGACAATTGAACGCGCGATGAAATTAATCGATGAGTATGATTTTTTATATGGCATTATC
    GGTTGGCATCCAGTTGACGCAATTGATTTTACAGAAGAACACCTGGAATGGATTGAATCT
    TTAGCTCAGCATCCAAAAGTGATTGGTATTGGTGAAATGGGATTAGATTATCACTGGGAT
    AAATCTCCTGCAGATGTTCAAAAGGAAGTTTTTAGAAAGCAAATTGCTTTAGCTAAGCGT
    TTGAAGTTACCAATTATCATTCATAACCGTGAAGCAACTCAAGACTGTATCGATATCTTA
    TTAGAGGAGCATGCTGAAGAGGTAGGCGGGATTATGCATAGCTTTAGTGGTTCTCCAGAA
    ATTGCAGATATTGTAACTAATAAGCTGAATTTTTATATTTCATTAGGTGGACCTGTGACA
    TTTAAAAATGCTAAACAGCCTAAAGAAGTTGCTAAGCATGTGTCAATGGAGCGTTTGCTA
    GTTGAAACCGATGCACCGTATCTCTCGCCACATCCGTATAGAGGGAAGCGAAATGAACCG
    GCGAGAGTAACTTTAGTAGCTGAACAAATTGCTGAATTAAAAGGCTTATCTTATGAAGAA
    GTGTGCGAACAAACAACTAAAAATGCAGAGAAATTGTTTAATTTAAATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    774

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS02590 [old locus tag: SA0449 ]
  • symbol: SA_RS02590
  • description: TatD family deoxyribonuclease
  • length: 257
  • theoretical pI: 4.90143
  • theoretical MW: 29281.1
  • GRAVY: -0.350584

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity hydrolase, TatD family (TIGR00010; HMM-score: 304)
  • TheSEED: see SA0449
  • PFAM:
    Amidohydrolase (CL0034) TatD_DNase; TatD related DNase (PF01026; HMM-score: 279)
    and 2 more
    Amidohydro_2; Amidohydrolase (PF04909; HMM-score: 20.7)
    Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 18.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001835
    • TAT(Tat/SPI): 0.000273
    • LIPO(Sec/SPII): 0.000448
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIERAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKSPADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPEIADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRNEPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS01275formate acetyltransferase  [2] (data from MRSA252)
    SA_RS01365L-lactate dehydrogenase  [2] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [2] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [2] (data from MRSA252)
    SA_RS04710hypothetical protein  [2] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [2] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [2] (data from MRSA252)
    SA_RS06490glutamine synthetase  [2] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [2] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [2] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [2] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [2] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [2] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [2] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [2] (data from MRSA252)
    SA_RS13735malate:quinone oxidoreductase  [2] (data from MRSA252)
    SA_RS13920arginine deiminase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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