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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06490 [old locus tag: SA1150 ]
  • pan locus tag?: SAUPAN003626000
  • symbol: SA_RS06490
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06490 [old locus tag: SA1150 ]
  • symbol: SA_RS06490
  • product: glutamine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1307165..1308505
  • length: 1341
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1307165..1308505) NCBI
  • BioCyc: SA_RS06490 BioCyc
  • MicrobesOnline: see SA1150

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAAAACGTACTTTCACTAAAGAAGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
    AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
    GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
    GGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGATTTAGATACTTGGGTA
    ATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATTTGTGATGTATATAAA
    ACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAACGTGTATTAAAAGAA
    ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCAGAATTCTTCTTGTTT
    AAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
    TTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
    ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGTCAACATGAAATTGAC
    TTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACATTTAAATTGGTTGTT
    AAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAACCATTATTCGGT
    GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
    TTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAATTTACAGCAGGTGTG
    CTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTAAACTCATATAAACGT
    TTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGTAAAAACCGTTCACCA
    TTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAAGTACGTTCAGTAGAT
    CCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCTGGACTAGATGGTATT
    AAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTACGAAATGAACCGTGAA
    GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTATACTGCATTAAAAGCA
    ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATCTATAATCAATTTATT
    AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTATCTGAATGGGAAAGAGAT
    CAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06490 [old locus tag: SA1150 ]
  • symbol: SA_RS06490
  • description: glutamine synthetase
  • length: 446
  • theoretical pI: 4.84142
  • theoretical MW: 50854.4
  • GRAVY: -0.438117

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamine synthetase ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 582.1)
    and 1 more
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED: see SA1150
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    and 2 more
    no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002004
    • TAT(Tat/SPI): 0.000426
    • LIPO(Sec/SPII): 0.000276
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPKRTFTKEDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY, GlnR see SA1150

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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