PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS03530 [old locus tag: SA0615 ]
• pan locus tag^?: SAUPAN002539000
• symbol: SA_RS03530
• pan gene symbol^?: graS
• synonym:
• product: sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS03530 [old locus tag: SA0615 ]
• symbol: SA_RS03530
• product: sensor histidine kinase
• replicon: chromosome
• strand: +
• coordinates: 708912..709952
• length: 1041
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (708912..709952) NCBI
• BioCyc: SA_RS03530 BioCyc
• MicrobesOnline: see SA0615

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  481
  541
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  901
  961
  1021
  
  ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGG
  ATATTGTTTTTAAACTTGCTTATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATA
  GACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAACAATGATTTTTCTTATATTG
  ACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
  ATTAAACATAAAGATTTAGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATAT
  CGTCAAATCTCAGCGCACAAAGAAAAGGTTGTTGAGCAACAGTTACAATTGAACATGCAT
  GAACAAACCATTACAGAATTTGTGCACGACATAAAAACACCTGTGACAGCCATGAAATTA
  TTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAGGCATTACTATATGAATGGTCTCGT
  ATAAACTCGATGCTGGATACACAGCTGTATATTACTAGATTAGAATCTCAACGCAAAGAT
  ATGTATTTTGATTACGTGTCACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGA
  CATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACTTTAAAGTGGATGATTATGTT
  TATACAGATACAAAATGGTGTCGTATGATTATTAGACAGATTTTGTCAAACGCATTGAAA
  TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTA
  TATATTAAAGACTATGGCAGAGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGA
  GGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTTCAGGTATGGGTCTATATTTA
  GTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
  GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAA
  GTGACAAATTTGTCATTTTAA
  60
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  1041
  

⊟Protein

⊟General

• locus tag: SA_RS03530 [old locus tag: SA0615 ]
• symbol: SA_RS03530
• description: sensor histidine kinase
• length: 346
• theoretical pI: 7.34071
• theoretical MW: 41032.2
• GRAVY: -0.248555

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 90.7)
  and 10 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 67.3)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 61)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.4)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.4)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 26.3)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 26.3)
  Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 22.5)
  Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 22.5)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 14.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 12.9)
• TheSEED: see SA0615
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 69.4)
  and 4 more
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 17.4)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.3)
  no clan defined TA0956; Thermoplasma acidophilum protein TA0956 (PF11513; HMM-score: 14.5)
  Corona_M; Coronavirus M matrix/glycoprotein (PF01635; HMM-score: 7.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008443
  • TAT(Tat/SPI): 0.000153
  • LIPO(Sec/SPII): 0.046968
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446984008 NCBI
• RefSeq: WP_001061264 NCBI
• UniProt: see SA0615

⊟Protein sequence

• MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDTKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications