PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS04225 [old locus tag: SA0742 ]
• pan locus tag^?: SAUPAN002779000
• symbol: SA_RS04225
• pan gene symbol^?: clfA
• synonym:
• product: clumping factor A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS04225 [old locus tag: SA0742 ]
• symbol: SA_RS04225
• product: clumping factor A
• replicon: chromosome
• strand: +
• coordinates: 848484..851453
• length: 2970
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (848484..851453) NCBI
• BioCyc: SA_RS04225 BioCyc
• MicrobesOnline: see SA0742

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCA
  GTGCTTGTAGGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGT
  GAAAATAGTGTTACGCAATCTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGT
  AGCGTTAGTGCTGCACCTAAAACAGACGACACAAACGTGAGTGATACTAAAACATCGTCA
  AACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAA
  TCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACG
  ACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAA
  TTAGTGAATCAAACAAGTAATGAAACGACTTCTAATGATACTAATACAGTATCATCTGTA
  AATTCACCTCAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACT
  GAAGCAACACCTTCAAACAATGAATCAGCTCCACAGAATACAGATGCAAGTAATAAAGAT
  GTAGTTAGTCAAGCGGTTAATCCAAGTACGCCTAGAATGAGAGCATTTAGTTTAGCGGCA
  GTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACGAATCAGTTGACAGATGTGAAA
  GTTACTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATGTCAAACTGAAT
  TATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACCT
  AAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGA
  GATCAAGTATTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACA
  GACTATGTTGATAATAAAGAAAATGTAACAGCTAATATTACTATGCCAGCTTATATTGAC
  CCTGAAAATGTTACAAAGACAGGTAATGTGACATTGACAACTGGCATAGGAACCAATACT
  GCTAGTAAGACAGTATTAATCGACTATGAGAAATATGGACAATTCCATAATTTATCAATT
  AAAGGTACGATTGATCAAATCGATAAAACAAATAATACGTATCGCCAAACAATTTATGTC
  AATCCAAGCGGAGATAACGTTGTGTTACCTGCCTTAACAGGTAATTTAATTCCTAATACA
  AAGAGTAATGCGTTAATAGATGCAAAAAACACTGATATTAAAGTTTATAGAGTCGATAAT
  GCTAATGATTTATCTGAAAGTTATTATGTGAATCCTAGCGATTTTGAAGATGTAACTAAT
  CAAGTTAGAATTTCATTTCCAAATGCTAATCAATACAAAGTAGAATTTCCTACGGACGAT
  GACCAAATTACAACACCGTATATTGTAGTTGTTAATGGCCATATTGATCCTGCTAGTACA
  GGTGATTTAGCACTACGTTCGACATTTTATGGTTATGATTCTAATTTTATATGGAGATCT
  ATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGAT
  AAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGAT
  TCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAT
  TCTGGCAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCA
  AGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGTGATTCAGATTCAGCAAGTGAT
  TCAGATTCAGCAAGTGATTCAGACTCAGCAAGTGATTCAGATTCAGCAAGTGATTCAGAT
  TCAGCAAGCGATTCAGATTCAGCGAGCGATTCAGATTCAGCGAGCGATTCAGATTCAGCG
  AGTGATTCCGACTCAGCGAGCGATTCAGACTCAGATAGTGACTCAGATTCCGATAGCGAT
  TCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCTGACTCAGACAGCGATTCTGAC
  TCAGACAGTGACTCAGATTCCGATAGCGATTCCGACTCAGACAGTGACTCAGATTCCGAT
  AGCGATTCCGACTCAGACAGTGACTCAGATTCCGATAGCGATTCAGATTCCGACAGTGAT
  TCCGACTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAT
  TCAGACAGCGATTCTGACTCAGACAGTGACTCAGATTCCGATAGCGATTCAGATTCAGAC
  AGTGATTCAGACTCAGATAGCGATTCAGATTCCGACAGTGACTCAGACTCAGACAGCGAT
  TCAGATTCCGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGATAGTGACTCGGAT
  TCAGCGAGTGATTCAGATTCAGATAGCGATTCAGAATCAGATAGTGACTCAGACTCAGAC
  AGTGATTCAGATTCAGATAGTGACTCAGACTCAGACAGCGATTCAGAATCAGATAGTGAC
  TCCGATTCAGACAGCGATTCAGAATCAGATAGTGACTCCGATTCAGATAGCGATTCGGAT
  TCAGCGAGTGATTCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCAGATTCCGAT
  TCAACGAGTGACACAGGATCAGACAACGACTCAGACAGTGATTCAAATAGCGATTCCGAG
  TCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGTACTAATGCTTCT
  AATAAAAATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGTTCTGAAGATGAA
  GCGAATACGTCACTAATTTGGGGATTATTAGCATCATTAGGTTCATTACTACTTTTCAGA
  AGAAAAAAAGAAAATAAAGATAAGAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS04225 [old locus tag: SA0742 ]
• symbol: SA_RS04225
• description: clumping factor A
• length: 989
• theoretical pI: 3.38508
• theoretical MW: 102408
• GRAVY: -1.1

⊟Function

• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 28.2)
  Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 27.8)
• TheSEED: see SA0742
• PFAM:
  Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 135.9)
  and 2 more
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 35)
  Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 25.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 39 aa
  • SP(Sec/SPI): 0.955997
  • TAT(Tat/SPI): 0.010304
  • LIPO(Sec/SPII): 0.029148
  • Cleavage Site: CS pos: 39-40. ADA-SE. Pr: 0.8951
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446978939 NCBI
• RefSeq: WP_001056195 NCBI
• UniProt: see SA0742

⊟Protein sequence

• MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQAVNPSTPRMRAFSLAAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANITMPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYVNPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRSTFYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKENKDKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications