PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05030 [old locus tag: SA0886 ]
• pan locus tag^?: SAUPAN003223000
• symbol: SA_RS05030
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05030 [old locus tag: SA0886 ]
• symbol: SA_RS05030
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1007337..1009301
• length: 1965
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1007337..1009301) NCBI
• BioCyc: SA_RS05030 BioCyc
• MicrobesOnline: see SA0886

⊟Phenotype

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⊟DNA sequence

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  ATGAAATGGTTCAAATTAATACTTGATGTTACAACCTTCATTCTGATTGCTATATTGTTA
  TTTGTTTATACATATAAAGAAAACGAAGAAATATTGCCTGATACTAAATACCCTATAGCG
  GTAACTGACTGGAATAAAAAATATAGTAAAAATGAGATTTATAAACGTATAAATCAATTC
  GCTAAAAATGAGAACGTAGCAATCTATAAATCAACTTCAAATTATACAAACAAAAACGTA
  GATAAAGATATATATGTATTTAATAAATCAAAAGCAACAACTATCACTCCTTTTAACGCT
  AAATATAACATTCATTATTTAAGTGACGACGAATTATTAAAAAAAGATATCAAAGGAAGT
  TATTTTGTAAAAGACAAAAATTTTGACGTGTCTAAATTCATAAATTTTTTAAAAGAATAT
  GGTGTTACTGCTGAATCATACAAAATAGATCATATGATGATTGCCGTTGGTGTCATTAAA
  CAAATGAATATAGAAGTTCCGTTATCCGCACTTTTAATCGTTTATTTTATTTATTATATT
  TTCGAAAAGAACATTAATTTCAAAGCGTATGCGATTAAGTATTTAAATGGTTTTACATTA
  AGAAAAATAATTTTTGAAAATTTTTCAAAAAAATGCACGTATTGGGTAACGTTAATCATA
  AGTCAAATTCTTTTAACTACAAGTGTACTTTGGATATTAAATTACACGGGTAATTTAGAT
  TTATTCATATTAAGATTAGTTCTGCTTTCATGTCTTTTCATTTTAACGATTAGTGTTATT
  AATTTATGGACTTTCTTAATGTTACTAAATTTAAATATTGCTAATATGATTAAAGGTAAG
  CAGCACTTTAAAACAATTCGTTTTATTAATACAGTTTGTAAAAGTATTCTCTTAGTACTA
  ATAGCTAGTGTAATGATAGAAAATACTAGTGTTATTAAAGATTTGAATAAAATAAAGGAA
  ACTGAGAAATATTGGAATGTATTAGATGATTATTACACGATTGAATTTGCACCTTATCAC
  GAAACGAAACAAAGTTTGATTGATAATATGCTGCGATCAGAACAATTAGTAAAGGCTAGT
  GAAGCAGAAAATAATGCGATTTTATTCAAACCAAAGGGTGACTCCGTTGACAATGACAAC
  TTTTCGCCTGATGAGGGGAATGTAATATTAGTAAATAATCAGTTCTGGTCGATTTATTAC
  AAGCAGTTTCAACCTGATATTCCGATAAAAAATCAAAAAAATAATGTCGAAGTAATTATT
  CCACAAAAGTTTCATGCAATGCGTAATGAAATCAATCAAGCATATCATTCATGGTTTGAA
  TTTGTACAAAATAAAAATAATAAAGAGAATAAGTTATCTATACAGTTTATCAACAAAAAT
  GATTATCGAATTTTTTCATTTGATGCACGAGATAGTCGCCATTTGTCATTTATAGAGGCG
  CCAATCATTGTGAATGTTCAGGCATCAGATTTATCGAATGATTTTTATTATGCCATGATC
  AGTCAAGGCGGGTATTTATTCAAAAATTATGACGCGCTAGTAAAAAATATTGAAAATTAT
  CATCTTGATGGGGAAATCAGTGGAATAACCAATTATAAAGATAGCGTGATGGAAATGTAT
  CATGAAAACAATTTGAAATTAACAGTACTCAACTTTTCACAAATCATTATCGCAATCATT
  TTAATAATTATTATTTTATTTGATGTGAAATATTATTTTGAACAGCATCGAAAATTACTC
  GTAATCAAAAAGCTATATGGTTATTCAACATTAAGAGCCAATTACCAATACTTATTAATA
  AATAATATAGTTGTTGTTTTTATTGGAATATTGACGAATGTAATTTTACATTCTCAGTAT
  ATAATGATGATATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTA
  TACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAA
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⊟Protein

⊟General

• locus tag: SA_RS05030 [old locus tag: SA0886 ]
• symbol: SA_RS05030
• description: hypothetical protein
• length: 654
• theoretical pI: 9.1671
• theoretical MW: 77055.3
• GRAVY: 0.0475535

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance bacteriocin-associated integral membrane protein (TIGR01654; HMM-score: 735.3)
• TheSEED: see SA0886
• PFAM:
  BPD_transp_1 (CL0404) DUF1430; Protein of unknown function (DUF1430) (PF07242; HMM-score: 48.9)
  and 2 more
  no clan defined DHHA2; DHHA2 domain (PF02833; HMM-score: 11.7)
  Trigger_C (CL0262) SurA_N_3; SurA N-terminal domain (PF13624; HMM-score: 8.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.152805
  • TAT(Tat/SPI): 0.000609
  • LIPO(Sec/SPII): 0.023157
• predicted transmembrane helices (TMHMM): 7

⊟Accession numbers

• GI: 446793573 NCBI
• RefSeq: WP_000870829 NCBI
• UniProt: see SA0886

⊟Protein sequence

• MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDWNKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKYNIHYLSDDELLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMNIEVPLSALLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIISQILLTTSVLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDYRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIENYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILHSQYIMMIFATILVVQILLQICSLYYHGRRFNEVIKEF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications