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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05115 [old locus tag: SA0902 ]
  • pan locus tag?: SAUPAN003254000
  • symbol: SA_RS05115
  • pan gene symbol?: patA
  • synonym:
  • product: N-acetyl-L,L-diaminopimelate aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS05115 [old locus tag: SA0902 ]
  • symbol: SA_RS05115
  • product: N-acetyl-L,L-diaminopimelate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1023203..1024357
  • length: 1155
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1023203..1024357) NCBI
  • BioCyc: SA_RS05115 BioCyc
  • MicrobesOnline: see SA0902

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAACTTTCTTTAAATTCTAATTCTAAATATTTAAGAGCACCAAGTATTCGCCAATTT
    TCAAATCGTATGAATAATTTGGATGATTGTGTTAATTTAACTATTGGCCAACCAGACTTC
    CCGATGCCTGATGTTGTGAAAAAGGCATATATTGATGCTATTAACAATGACAAGACATCA
    TACTCTCACAATAAAGGGTTATTAGAAACTCGCGAAGCAATTAGTCAATACTTCAAAAAT
    CGTTATCATTTTTCCTATGATCCTGAAGAAATTATTGTGACAAATGGAGCAAGTGAAGCA
    ATAGACACAACGTTACGTTCTATCATAGAGCCTGGAGATGAAATTATAATACCGGGACCA
    ATTTATGCAGGCTACATACCACTCATCGAAGTACTAGGTGGTAAACCAATTTATATTGAT
    ACAACAGCAACACAATTCAAAATTACACCTGATGCATTAGAAAGTCATATTTCTCCAAAG
    ACAAAAGCTGTCTTGTTAAATTATCCGACTAATCCAACTGGCGTAGTTTTAAAAAGAAAT
    GAAGTTTTAAATATCGTAAATGTATTAAAAAAATATCCGATATTTATTATTAGCGATGAG
    ATTTATGCTGAAAATACATTTAGTGGTAAACATGTATCCTTCGCTGAATTTGAAGACATT
    CGTGATCAATTAATATTAATTGGTGGTTTAAGTAAATCACACTCAGCAACCGGAATAAGA
    ATTGGTTTTCTATTAGGGCCTCAATATTTGATTGATAAATTAACATTTATGCACGCCTAT
    AATTGTATTTGTGCCAATGTTCCAGCTCAAATAGCATGTATAACAGCACTTAACGAAGGA
    TTAGAAGCTCCTAAATATATGAACGAAGCTTATGTAGAACGAAGAAATTATTTAGTATCT
    GAATTAACTAAATTAGGTTTTGAGATTACCGCTCAACCTGAAGGCGCGTTTTATATTTTC
    CCAAGTATTAAACATATCACCGATGACGATTTCGAATTTTGTGTCGATTTACTTGAATCA
    ACACATTTAGCAATCGTTCCAGGCTCATCCTTTACAGAATTCGGTAAAGGATTTGTAAGG
    ATTTCCTATGCTTATGAAATGGATGTTTTAAAAGAAGGTATGAAGAGACTTGCTAAATAT
    TTAAATACTAAATAA
    60
    120
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    240
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    360
    420
    480
    540
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    660
    720
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    840
    900
    960
    1020
    1080
    1140
    1155

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS05115 [old locus tag: SA0902 ]
  • symbol: SA_RS05115
  • description: N-acetyl-L,L-diaminopimelate aminotransferase
  • length: 384
  • theoretical pI: 5.66622
  • theoretical MW: 43146.2
  • GRAVY: -0.114323

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.-?  ExPASy
  • TIGRFAM:
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 211.3)
    and 31 more
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 157.8)
    succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 138.6)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 136.6)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 128.9)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 127.6)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 124)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 123.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 119.8)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 102.3)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 100.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 86.2)
    enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 54.6)
    Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 38.9)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 28.8)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides TDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 26.5)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 25.9)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 24.8)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 22.8)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 18.7)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 18.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.5)
    aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 16.2)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 16)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 14.4)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 14.3)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 13.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 13)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 12.6)
    Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 11.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 11.8)
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 11)
  • TheSEED: see SA0902
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 213)
    and 4 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 39.4)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 36.3)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 35.4)
    OKR_DC_1; Orn/Lys/Arg decarboxylase, major domain (PF01276; HMM-score: 18.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010565
    • TAT(Tat/SPI): 0.000464
    • LIPO(Sec/SPII): 0.001211
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPDVVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAIDTTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPKTKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFEDIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITALNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFCVDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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