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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS05395 [old locus tag: SA0953 ]
- pan locus tag?: SAUPAN003326000
- symbol: SA_RS05395
- pan gene symbol?: potD
- synonym:
- product: spermidine/putrescine ABC transporter substrate-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGAAACGTTTTTTACAACTCATTATAGGTGCATTAGTTGTGGGTATGCTTTGTCTTACT
TTAAGTCATTGGTTTAAATCTAAAGAACAAGTGCATACAAATCAAAAAATTTACGTATAC
AATTGGGGCGAATATATTGATCCAGAGTTAATTAAGAAATTTGAAAAAGAAACTGGCATT
CAAGTCGTTTATGAAACTTTCGATTCAAATGAAGCGATGGAAGCCAAAATTCGCAATGGC
GGTACACATTATGATGTTGCTTTTCCTAGTGAATATACAGTTCAAAAATTGAAAAGAGAT
CATTTATTGTTACCAATAGATCATAATAAGGTACCTAATATTAAAAATTTAGATTCAGAT
TATATGAATATGTCATTTGATAAAGGCAATAAATATTCTTTACCTTATTTCTTTGGAACT
GTAGGTATTTTATATAATAAAGAAAAGTATCCAAATGAATCATTTGATAGTTGGAAGTCA
TTGTATAATCCTAAATTTAAAAACCAAATTTTACTAGTTGACGGTGCTAGAGAGATTATA
GGCATGAGTTTGAATAAACTTGGGTATAATCTTAATGACCGTAATTCGCACCATTTAAAA
GAAGCAGAGCGAGATTTAACCAAACTAGCACCACAAGTAAGAGGTGTCGTAGGTGATGAA
ATTACCATGATGCTTCAACAAAATGAAGGTAACATAGCGGTTGTTTGGAGTGGTGTTGCA
GCACCTCTAGTGCAAGAAGGGGATAAATATAATTATGTTATTCCTAAAGAAGGATCGAAT
TTATGGTTCGACAATATGGTAATTCCAAAAACGGCACAAAATAAAGAGGGTGCATATAAA
TTTATGAATTTTTTATTAGATGCTAAAAATAACAAGCAAAATACAGAATTCGTAGGCTAT
GCAACGCCAAACAAGGCTGCTCGACAATTGTTGCCTAAAGAGATTAAAGACGACCATCGT
TTTTATCCGACTAAGAAAGAGCAAGAACGCCTTGAAGTTTATAAAGATTTAGGACCTGAA
GTTTTAAGTGAATACAATGAAAACTTTTTGAATTTCAAAATGTCATTAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS05395 [old locus tag: SA0953 ]
- symbol: SA_RS05395
- description: spermidine/putrescine ABC transporter substrate-binding protein
- length: 357
- theoretical pI: 8.29013
- theoretical MW: 41443.1
- GRAVY: -0.582073
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 23)and 4 moreTransport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 16.1)Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 16.1)Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 16)Transport and binding proteins Other thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein (TIGR01276; HMM-score: 13.7)
- TheSEED: see SA0953
- PFAM: PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 125.3)SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 102.7)and 5 moreSBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 74.6)SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 53.4)Lipoprotein_8; Hypothetical lipoprotein (MG045 family) (PF02030; HMM-score: 32.6)ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 16.9)PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helix: 1
- LocateP:
- SignalP: Signal peptide SP(Sec/SPI) length 33 aa
- SP(Sec/SPI): 0.49937
- TAT(Tat/SPI): 0.004282
- LIPO(Sec/SPII): 0.241175
- Cleavage Site: CS pos: 33-34. VHT-NQ. Pr: 0.1597
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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