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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05395 [old locus tag: SA0953 ]
  • pan locus tag?: SAUPAN003326000
  • symbol: SA_RS05395
  • pan gene symbol?: potD
  • synonym:
  • product: spermidine/putrescine ABC transporter substrate-binding protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS05395 [old locus tag: SA0953 ]
  • symbol: SA_RS05395
  • product: spermidine/putrescine ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1079566..1080639
  • length: 1074
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1079566..1080639) NCBI
  • BioCyc: SA_RS05395 BioCyc
  • MicrobesOnline: see SA0953

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACGTTTTTTACAACTCATTATAGGTGCATTAGTTGTGGGTATGCTTTGTCTTACT
    TTAAGTCATTGGTTTAAATCTAAAGAACAAGTGCATACAAATCAAAAAATTTACGTATAC
    AATTGGGGCGAATATATTGATCCAGAGTTAATTAAGAAATTTGAAAAAGAAACTGGCATT
    CAAGTCGTTTATGAAACTTTCGATTCAAATGAAGCGATGGAAGCCAAAATTCGCAATGGC
    GGTACACATTATGATGTTGCTTTTCCTAGTGAATATACAGTTCAAAAATTGAAAAGAGAT
    CATTTATTGTTACCAATAGATCATAATAAGGTACCTAATATTAAAAATTTAGATTCAGAT
    TATATGAATATGTCATTTGATAAAGGCAATAAATATTCTTTACCTTATTTCTTTGGAACT
    GTAGGTATTTTATATAATAAAGAAAAGTATCCAAATGAATCATTTGATAGTTGGAAGTCA
    TTGTATAATCCTAAATTTAAAAACCAAATTTTACTAGTTGACGGTGCTAGAGAGATTATA
    GGCATGAGTTTGAATAAACTTGGGTATAATCTTAATGACCGTAATTCGCACCATTTAAAA
    GAAGCAGAGCGAGATTTAACCAAACTAGCACCACAAGTAAGAGGTGTCGTAGGTGATGAA
    ATTACCATGATGCTTCAACAAAATGAAGGTAACATAGCGGTTGTTTGGAGTGGTGTTGCA
    GCACCTCTAGTGCAAGAAGGGGATAAATATAATTATGTTATTCCTAAAGAAGGATCGAAT
    TTATGGTTCGACAATATGGTAATTCCAAAAACGGCACAAAATAAAGAGGGTGCATATAAA
    TTTATGAATTTTTTATTAGATGCTAAAAATAACAAGCAAAATACAGAATTCGTAGGCTAT
    GCAACGCCAAACAAGGCTGCTCGACAATTGTTGCCTAAAGAGATTAAAGACGACCATCGT
    TTTTATCCGACTAAGAAAGAGCAAGAACGCCTTGAAGTTTATAAAGATTTAGGACCTGAA
    GTTTTAAGTGAATACAATGAAAACTTTTTGAATTTCAAAATGTCATTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1074


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS05395 [old locus tag: SA0953 ]
  • symbol: SA_RS05395
  • description: spermidine/putrescine ABC transporter substrate-binding protein
  • length: 357
  • theoretical pI: 8.29013
  • theoretical MW: 41443.1
  • GRAVY: -0.582073

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 23)
    and 4 more
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 16.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids carbohydrate ABC transporter substrate-binding protein, CPR_0540 family (TIGR03850; HMM-score: 16.1)
    Metabolism Transport and binding proteins Other ABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 16)
    Metabolism Transport and binding proteins Other thiamin/thiamin pyrophosphate ABC transporter, thiamin/thiamin pyrophospate-binding protein (TIGR01276; HMM-score: 13.7)
  • TheSEED: see SA0953
  • PFAM:
    PBP (CL0177) SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 116.9)
    and 7 more
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 82.3)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 77.1)
    SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 51.8)
    Lipoprotein_8; Hypothetical lipoprotein (MG045 family) (PF02030; HMM-score: 33.4)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 17.2)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 14.4)
    PH (CL0266) FERM_C; FERM C-terminal PH-like domain (PF09380; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0003
    • Cytoplasmic Membrane Score: 0.9007
    • Cell wall & surface Score: 0.002
    • Extracellular Score: 0.0971
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 33 aa
    • SP(Sec/SPI): 0.49937
    • TAT(Tat/SPI): 0.004282
    • LIPO(Sec/SPII): 0.241175
    • Cleavage Site: CS pos: 33-34. VHT-NQ. Pr: 0.1597
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGEYIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHLLLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

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