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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07225 [old locus tag: SA1275 ]
  • pan locus tag?: SAUPAN003897000
  • symbol: SA_RS07225
  • pan gene symbol?:
  • synonym:
  • product: PepSY domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07225 [old locus tag: SA1275 ]
  • symbol: SA_RS07225
  • product: PepSY domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1480471..1481811
  • length: 1341
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1480471..1481811) NCBI
  • BioCyc: SA_RS07225 BioCyc
  • MicrobesOnline: see SA1275

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAAATACATTTAATCCATTACAAAGATTACATTTCTATGCAGCAATATTTATTGCT
    CCACTGTTAATCACTTTAACCATTTCGGGCATTGGTTACTTGTTCTTTCCAGAAGTTGAA
    AATAATATTTATAAGAATGAGTTTTTTGGTGACAGTGATGTAAAAACGCATCAAACATTA
    AATGATGCAGTACATCAAGTTGAACAACAATATGAAGGATTCTTTGTAAGTAAAGTTAGC
    ATACTTGATGAACCATATAACAAACGAATTACACTGAGTGATATGGCAGGAAATCAACGT
    TACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTAT
    TCTAATGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTAT
    TTAGTAGAGTTAACCGCATGTTGGACGATATTCATGATTTTATCTGGTACTTATTTACTC
    ATTAAGAAGCATTTAATTTCTAACAAAAGTAAGGCACTTCGTTGGCAAAAGTGGCACGCA
    ATGATTGGAGTTATCATTGCAATTCCAGTATTTGTATTAGTTTTAACTGGATTGCCATGG
    TCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGACCTTGGTCAA
    GGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGT
    AAAAATAAACAGCCAGCTTCATCCGAAAAAGGTTCAAGTGGTCATCATGGTAATGCAGCA
    ATGCCTCAAACCAAATTAGATTATCAAATATCTATTGATAAGGTCGTTGTACAAGCGCAA
    AAAGCTGGTATTAAAAAGCCGTTTTCAATCGTATATCCAAGTGATAAAAATGGTACCTTT
    ATTGTATCTAATACTAGTAATTCAGGTGTTACTGGGCTAGATGTATCACCTTACAAGGAA
    CAAACACTTTATTTCGACCAATATAGCGGTAAAAAGCTAGGTACGATTAAATATGATGAC
    TACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTC
    GGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCACTTTATTAGTAGCCATTGGC
    ATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAGTAAAAGTACCACATCGC
    GTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATG
    CCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAA
    GATCGTCGTGCTAAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS07225 [old locus tag: SA1275 ]
  • symbol: SA_RS07225
  • description: PepSY domain-containing protein
  • length: 446
  • theoretical pI: 9.82061
  • theoretical MW: 50171.2
  • GRAVY: 0.0807175

Function[edit | edit source]

  • TIGRFAM:
    TIGR03943 family protein (TIGR03943; HMM-score: 10.6)
  • TheSEED: see SA1275
  • PFAM:
    PepSY_TM-like (CL0490) PepSY_TM; PepSY-associated TM region (PF03929; HMM-score: 172.7)
    and 3 more
    no clan defined Peptidase_A8; Signal peptidase (SPase) II (PF01252; HMM-score: 9.8)
    TctB; Tripartite tricarboxylate transporter TctB family (PF07331; HMM-score: 8)
    GAPT; GRB2-binding adapter (GAPT) (PF11770; HMM-score: 6.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.123574
    • TAT(Tat/SPI): 0.000974
    • LIPO(Sec/SPII): 0.036904
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIYKNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYVFLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLLIKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDLGQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVVVQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLGTIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCITLLVAIGMGFVSWIKRTKNTAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]