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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07635 [old locus tag: SA1347 ]
  • pan locus tag?: SAUPAN004071000
  • symbol: SA_RS07635
  • pan gene symbol?: bfmBAB
  • synonym:
  • product: alpha-ketoacid dehydrogenase subunit beta

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07635 [old locus tag: SA1347 ]
  • symbol: SA_RS07635
  • product: alpha-ketoacid dehydrogenase subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 1556807..1557790
  • length: 984
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_002745 (1556807..1557790) NCBI
  • BioCyc: G1G21-1536 BioCyc
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTAAATTATCTTATTTAGAGGCGATACGCCAGGCACAAGACTTAGCATTACAACAA
    AATAAAGATGTGTTTATTCTTGGTGAAGATGTTGGTAAAAAAGGTGGCGTATTTGGTACT
    ACTCAAGGACTACAACAACAATATGGTGAAGACAGAGTTATCGATACGCCATTAGCTGAG
    TCAAATATTGTTGGAACTGCCATTGGTGCAGCTATGGTAGGTAAAAGACCAATTGCAGAA
    ATTCAATTTGCAGATTTTATTTTACCTGCGACAAATCAAATTATTAGTGAAGCTGCGAAA
    ATGCGTTATCGTTCAAATAATGATTGGCAATGCCCATTAACGATTCGTGCACCATTTGGT
    GGCGGTGTTCACGGAGGACTATATCATTCACAAAGTATTGAAAGTATATTTGCTTCATCA
    CCAGGTTTAACAATTGTTATTCCTTCCACACCGTATGATGCTAAAGGGCTTTTATTATCA
    TCTATCGAATCAAATGATCCAGTATTGTACTTTGAGCATAAAAAAGCTTATCGTTTCTTA
    AAGGAAGAAGTGCCAGAAGAATATTACACTGTACCTTTAGGAAAAGCAGATGTTAAACGT
    GAAGGTGAAGATTTAACAGTATTTTGTTATGGATTAATGGTCAATTATTGTTTACAAGCA
    GCGGATATTTTAGCTGCAGACGGTATCAACGTTGAAGTCGTTGATTTACGAACTGTTTAT
    CCATTAGATAAAGAAACAATTATTGATCGTGCTAAAAATACAGGTAAAGTTTTACTTGTA
    ACTGAAGATAATTTAGAAGGTAGTATTATGTCAGAAGTGTCAGCGATTATTGCAGAGCAT
    TGCTTGTTCGATTTAGATGCACCAATCATGCGTTTAGCTGCTCCAGATGTACCATCTATG
    CCATTTTCTCCTGTATTAGAAAATGAAATTATGATGAATCCAGAAAAAATCTTAAATAAA
    ATGCGTGAATTAGCAGAATTCTAG
    60
    120
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    984

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS07635 [old locus tag: SA1347 ]
  • symbol: SA_RS07635
  • description: alpha-ketoacid dehydrogenase subunit beta
  • length: 327
  • theoretical pI: 4.42709
  • theoretical MW: 36010.1
  • GRAVY: -0.0422018

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 75.9)
    and 2 more
    2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 23.7)
    indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 20.6)
  • TheSEED:
  • PFAM:
    THDP-binding (CL0254) Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 155.5)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 136.1)
    and 2 more
    PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 24.1)
    no clan defined DUF2795; Protein of unknown function (DUF2795) (PF11387; HMM-score: 12.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA_RS11130(deoA)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SA_RS09855(gatA)glutamyl-tRNA(Gln) amidotransferase subunit A  [1] (data from MRSA252)
    SA_RS00310aminoglycoside O-nucleotidyltransferase ANT(4')-Ia  [1] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [1] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [1] (data from MRSA252)
    SA_RS02955elongation factor G  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04420ABC transporter ATP-binding protein  [1] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS04710hypothetical protein  [1] (data from MRSA252)
    SA_RS04915enoyl-ACP reductase  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS05860cell division protein FtsZ  [1] (data from MRSA252)
    SA_RS05945carbamoyl-phosphate synthase large chain  [1] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [1] (data from MRSA252)
    SA_RS07205serine/threonine dehydratase  [1] (data from MRSA252)
    SA_RS076302-oxoglutarate dehydrogenase E2  [1] (data from MRSA252)
    SA_RS076402-oxoisovalerate dehydrogenase subunit alpha  [1] (data from MRSA252)
    SA_RS07645dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SA_RS08560pyruvate kinase  [1] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [1] (data from MRSA252)
    SA_RS09850aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B  [1] (data from MRSA252)
    SA_RS11060aldehyde dehydrogenase family protein  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1169530S ribosomal protein S8  [1] (data from MRSA252)
    SA_RS13340pyruvate oxidase  [1] (data from MRSA252)
    SA_RS13375hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)
    SA_RS13920arginine deiminase  [1] (data from MRSA252)

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00726
    • TAT(Tat/SPI): 0.000337
    • LIPO(Sec/SPII): 0.00042
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 447019377 NCBI
  • RefSeq: WP_001096633 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGLQQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSSIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]