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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS08235 [old locus tag: SA1461 ]
- pan locus tag?: SAUPAN004240000
- symbol: SA_RS08235
- pan gene symbol?: apt
- synonym:
- product: adenine phosphoribosyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGGATTTAAAGCAATACGTATCAGAAGTTCAAGATTGGCCGAAACCAGGTGTTAGTTTC
AAGGATATTACTACAATTATGGATAATGGTGAAGCATATGGCTATGCAACAGATAAAATT
GTAGAATACGCAAAAGACAGAGATGTTGATATCGTTGTAGGACCTGAAGCGCGTGGCTTT
ATCATTGGCTGTCCTGTAGCTTATTCAATGGGGATTGGCTTTGCACCTGTTAGAAAAGAA
GGGAAATTACCTCGTGAAGTCATTCGTTATGAGTATGACCTAGAATATGGTACAAATGTT
TTAACAATGCACAAAGATGCAATTAAACCAGGTCAACGTGTGTTAATTACAGATGATTTA
TTAGCTACTGGTGGTACGATTGAAGCAGCAATAAAATTAGTTGAAAAATTAGGCGGTATC
GTAGTAGGTATTGCATTTATAATTGAATTGAAATATTTAAATGGTATTGAAAAAATTAAA
GATTACGATGTTATGAGTTTAATCTCATACGACGAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS08235 [old locus tag: SA1461 ]
- symbol: SA_RS08235
- description: adenine phosphoribosyltransferase
- length: 172
- theoretical pI: 4.48452
- theoretical MW: 19116.9
- GRAVY: -0.030814
⊟Function[edit | edit source]
- reaction: EC 2.4.2.7? ExPASyAdenine phosphoribosyltransferase AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
- TIGRFAM: Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 230.7)and 9 morePurines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis pur operon repressor PurR (TIGR01743; HMM-score: 47.6)Regulatory functions DNA interactions pur operon repressor PurR (TIGR01743; HMM-score: 47.6)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 44.1)Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 37.2)Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides xanthine phosphoribosyltransferase (TIGR01744; EC 2.4.2.22; HMM-score: 35.5)Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 27.9)Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uracil phosphoribosyltransferase (TIGR01091; EC 2.4.2.9; HMM-score: 19.3)Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 14.6)Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 13.3)
- TheSEED: see SA1461
- PFAM: PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 53.7)and 1 moreUPRTase; Uracil phosphoribosyltransferase (PF14681; HMM-score: 19.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.018018
- TAT(Tat/SPI): 0.000238
- LIPO(Sec/SPII): 0.001122
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYAKDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLTMHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIKDYDVMSLISYDE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SA_RS01960 acetyl-CoA acyltransferase [1] (data from MRSA252) SA_RS04895 NAD(+) kinase [1] (data from MRSA252) SA_RS06225 30S ribosomal protein S2 [1] (data from MRSA252) SA_RS06295 translation initiation factor IF-2 [1] (data from MRSA252) SA_RS07060 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)