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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08240 [old locus tag: SA1462 ]
  • pan locus tag?: SAUPAN004241000
  • symbol: SA_RS08240
  • pan gene symbol?: recJ
  • synonym:
  • product: single-stranded-DNA-specific exonuclease RecJ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08240 [old locus tag: SA1462 ]
  • symbol: SA_RS08240
  • product: single-stranded-DNA-specific exonuclease RecJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1668794..1671067
  • length: 2274
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1668794..1671067) NCBI
  • BioCyc: G1G21-1660 BioCyc
  • MicrobesOnline: see SA1462

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
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    1021
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    1141
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    ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAA
    TTAACATCAAAATTAAAGTTAACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATT
    ATTGATGAACAAGCAATTGAATCCATCATTTCAGACACAGATGTCAATCATGATGCATTG
    CAATTGAGCGATATGACTAAGGCTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
    AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTG
    ACAACATTACAACTTTTAGGTGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAA
    GGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATGATGAAGGCATAACATTAATC
    ATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
    GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCA
    ATCGTACATCCAATGCATCCATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGT
    GTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTACCTGATTATTTTAAAGCATTA
    GTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
    AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAA
    GAAGCGGGATACAATGACAATATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGA
    CTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTTGCGAATTGTTGATGACTGAC
    GACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
    GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAG
    GGAGATTTATTTTTACTTTTAGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTT
    GCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAATTTTAAATATTGATAGGGAG
    CAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
    TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACG
    ATGGATATTGAAAATATAGAATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTA
    TCTAAAACAACTTCGTTAGATCCCGTAAAGCCAGTAGACGTATTATTAACAGAAAATGAT
    ATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
    AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATCGGTCAACAA
    AAAAATCATCTAAAATTGACACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAAT
    GGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTAATATATTAGGATCTGTTCAA
    ATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAT
    GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGAT
    GAAAATATTGTCGTGCTTATTCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTAT
    TATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTATTAAGAGATTTACCAACGTCA
    ATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
    CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGT
    TATAAAGCATTAATAACTAAACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGT
    CAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGTTGAAAGTTTTCTTAGACTTA
    AAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
    ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTA
    TTATATCAAGATTTTTCAGAAATAAAAAATTGGATAAAGTCACAATTGTCGTGA
    60
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    2274

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08240 [old locus tag: SA1462 ]
  • symbol: SA_RS08240
  • description: single-stranded-DNA-specific exonuclease RecJ
  • length: 757
  • theoretical pI: 4.87326
  • theoretical MW: 85555.2
  • GRAVY: -0.285469

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair single-stranded-DNA-specific exonuclease RecJ (TIGR00644; EC 3.1.-.-; HMM-score: 643.2)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification pseudouridine synthase (TIGR00093; EC 5.4.99.-; HMM-score: 13.5)
  • TheSEED: see SA1462
  • PFAM:
    no clan defined ssDNA-exonuc_C; Single-strand DNA-specific exonuclease, C terminal domain (PF10141; HMM-score: 195.8)
    and 2 more
    DHHA1; DHHA1 domain (PF02272; HMM-score: 76.7)
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 49)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001833
    • TAT(Tat/SPI): 0.000118
    • LIPO(Sec/SPII): 0.000251
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDVNHDALQLSDMTKAIERIKRAIANDEKILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLGVDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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