PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• pan locus tag^?: SAUPAN005009000
• symbol: SA_RS10060
• pan gene symbol^?: eap/map
• synonym:
• product: protein map

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• symbol: SA_RS10060
• product: protein map
• replicon: chromosome
• strand: -
• coordinates: 2003333..2004763
• length: 1431
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2003333..2004763) NCBI
• BioCyc: SA_RS10060 BioCyc
• MicrobesOnline: see SA1751

⊟Phenotype

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⊟DNA sequence

• 1
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  481
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  661
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGAAATTTAAGTCATTGATTACAACAACATTAGCATTAGGCGTTATAGCATCAACAGGA
  GCAAACTTTAATACTAACGAAGCATCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
  ACATTACACCATGGACATTCTAACATCCAGATTCCATATACAATTACTGTGAACGGTACA
  AGCCAAAACATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
  ATAGAGAATAAAGTTAAATCAGTTTTATACTTTAATAGAGGTATTAGTGATATCGATTTA
  AGACTTTCAAAGCAAGCGGAATATACGGTTCATTTTAAAAATGGAACAAAAAGAGTTATC
  GATTTGAAATCAGGTATCTACACAGCTGACTTAATCAATACAAGTGACATTAAAGCTATC
  AGTGTTAACGTAGATACTAAAAAGCAACCTAAAGATAAAGCTAAAGCAAATGTTCAAGTG
  CCATATACAATCACAGTGAACGGCACAAGCCAAAACATTTTATCAAACCTAACATTTAAT
  AAAAATCAAAATATTAGTTACAAAGATTTAGAGGGTAAAGTTAAATCAGTTTTAGAATCA
  AATAGAGGTATTACTGATGTTGATTTAAGACTTTCGAAGCAAGCGAAATATACAGTTAAT
  TTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCGAATTTA
  ATCAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACATATCGAA
  AATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATCTAAATGGTACATCTACA
  AACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAATTTAACT
  AGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTAAAATAT
  GCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTACAGTTA
  AATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATTGAAATT
  ACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATTGCAGTA
  AATGGCACATCAACACCAATTTTATCAAAACTAAAAATTTCGAATAAACAATTAATTAGT
  TACAAATATTTAAACGACAAAGTGAAATCTGTATTAAAAAGTGAAAGAGGTATCAGTGAT
  CTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTATATTTCAAAAATGGAAAGAAA
  CAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAATTTATTTAGTGCCAAAGATATT
  AAAAAGATTGATATTGATGTAAAACAATACACTAAATCAAAAAAAAAATAA
  60
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  1431
  

⊟Protein

⊟General

• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• symbol: SA_RS10060
• description: protein map
• length: 476
• theoretical pI: 10.5909
• theoretical MW: 53376.8
• GRAVY: -0.552731

⊟Function

• TIGRFAM:
  modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 12.5)
• TheSEED: see SA1751
• PFAM:
  Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 466)
  and 10 more
  Tudor (CL0049) LBR_tudor; Lamin-B receptor of TUDOR domain (PF09465; HMM-score: 20.8)
  no clan defined DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 18.5)
  Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 17.9)
  Sm-like (CL0527) LSM14; Scd6-like Sm domain (PF12701; HMM-score: 14)
  no clan defined GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 13.6)
  HMA; Heavy-metal-associated domain (PF00403; HMM-score: 11.7)
  Trefoil (CL0066) DUF569; Domain of unknown function (DUF569) (PF04601; HMM-score: 11.7)
  no clan defined Ribosomal_S21e; Ribosomal protein S21e (PF01249; HMM-score: 11.2)
  Cupin (CL0029) VIT_2; Vault protein inter-alpha-trypsin domain (PF13757; HMM-score: 8.3)
  no clan defined Neisseria_TspB; Neisseria meningitidis TspB protein (PF05616; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.89345
  • TAT(Tat/SPI): 0.004555
  • LIPO(Sec/SPII): 0.096731
  • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5840
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 486219380 NCBI
• RefSeq: WP_001557458 NCBI
• UniProt: see SA1751

⊟Protein sequence

• MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SA1751

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications