From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS10425 [old locus tag: SA1815 ]
  • pan locus tag?: SAUPAN005205000
  • symbol: SA_RS10425
  • pan gene symbol?: ktrB
  • synonym:
  • product: potassium transporter KtrB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS10425 [old locus tag: SA1815 ]
  • symbol: SA_RS10425
  • product: potassium transporter KtrB
  • replicon: chromosome
  • strand: +
  • coordinates: 2054835..2056142
  • length: 1308
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2054835..2056142) NCBI
  • BioCyc: SA_RS10425 BioCyc
  • MicrobesOnline: see SA1815

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAACAAAGTCCACAAACCTTTATATTTTTATTTAATGCTTTTCTTCTCTACAACTATC
    ATAGGTGCTCTTTTATTATATTTACCTTTCACTGGTAAAAAGCCAATATCTTTTTTAGAT
    GCCCTATTTATAGCTTCAAGTGCATTTACAGTTACTGGCCTATCCCCAGTTGATATAGGA
    TCACAGTTTAATATACTTGGTGAAATAGTAATACTATTATTAATTCAAATAGGTGGTCTG
    GGTATCGTGACCGTAACCCTATTGACACTAGTATTTTTAAATAGAAAGATATCAATGAAA
    AATAGATTCTTGATTATGGTTACATGGAATATTGACGAACCTGGTGGTGTTATTAAGCTA
    ATTAAACACTTGGCTATTTATAGTTTAGTCACTGAATTAATTGGTATGATTTGTTTGTGT
    TTATCTTTTATACCAAAATTTGGTATAGGCAAAGGTTTATTTTTAAGCTTATTCACATCA
    GTATCAGCTTTTAATAATGCTGGATTTGCCCTTTTTAAGAATAACTTAATAGATTATTCT
    AGTGATCCAATTGTCATTATTACAATCTCAATACTCATAATATTTGGAGGTATTGGACAT
    TTTGTCGTAATAGACTTTATTAATTGTAAAAAATTGAGTAAATTATCTTTACATTCTAAA
    TTAGTCTTAACTACAACTAGTATCCTAATAATTATAGGAGCTATTACATTCTTTTTATTA
    GAACAGTTTAATACTATGCAACATATGGGACTAGTTGAAAAAATCGGAAATTCTTTTTTC
    CAATCAGTAACAACACGAACAGCGGGTTTTAACAGTATTGATATAGCAAGCATTAACAAA
    TCTACCGCCTTAATGTTAATGTTACTTATGTTTATTGGTGGTGCCCCTCTCAGTGCAGCT
    GGAGGAATTAAAATAACTACTTTTGCAGTGGCGTTTATATTTGTTTTAAATTATATACGT
    AAAGAAAATAATGTTTCAGTATTCAATAAAGAAATATCTGACAAACATATAAAACTATCT
    ATTGTTACCATTAATATCTCATTTCTATTTATCAGTATCATTACTTTTATATTATCGATA
    ATTAATCCGAACATATCATTAATCAAGTTATTATTCGAAGTGGTTTCTGCATTCGGAACA
    GTCGGGTTAAGTATGAACCTTACCACAGAATATCATGGTATTACTAAAATAATTATTATA
    TTCGTTATGCTTTGCGGTAAAGTAGGACTATTAACTTTATTAAGAACATTTATACCACCA
    AAAAGTCCTAAAAATTACCGCTACACTAAAGGACAAATTTATCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS10425 [old locus tag: SA1815 ]
  • symbol: SA_RS10425
  • description: potassium transporter KtrB
  • length: 435
  • theoretical pI: 10.2528
  • theoretical MW: 47978.5
  • GRAVY: 0.914713

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, TrkH family (TIGR00933; HMM-score: 330.4)
    and 1 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, Trk family (TIGR00934; HMM-score: 95.2)
  • TheSEED: see SA1815
  • PFAM:
    Ion_channel (CL0030) TrkH; Cation transport protein (PF02386; HMM-score: 177.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.069376
    • TAT(Tat/SPI): 0.001357
    • LIPO(Sec/SPII): 0.027944
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFIASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS10425&oldid=96331"