From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS10650 [old locus tag: SA1855 ]
  • pan locus tag?: SAUPAN005299000
  • symbol: SA_RS10650
  • pan gene symbol?: rimI
  • synonym:
  • product: ribosomal-protein-alanine N-acetyltransferase RimI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS10650 [old locus tag: SA1855 ]
  • symbol: SA_RS10650
  • product: ribosomal-protein-alanine N-acetyltransferase RimI
  • replicon: chromosome
  • strand: -
  • coordinates: 2095540..2095950
  • length: 411
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2095540..2095950) NCBI
  • BioCyc: SA_RS10650 BioCyc
  • MicrobesOnline: see SA1855

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    GTGCCACAAGTCTTTGATCTTGAGCGTCGGAGTTTCAATGACAGTTCTTGGACAATTGAC
    GCATTTTATCATGAAATAGAGCAAAATAACTTTGCGAAATATTTTGTCTTAGAATTTGAA
    CAACAAATTATTGGTTATTTAGGTTTATGGATTGTTATCGATCAAGCTCAAATTACAACA
    GTTGCAATCGATGATAATTATAGAGGCTATGGTTTAGGTCAAATGCTTTTAAAATACGGA
    ATAAACTATGCAAGTCACACTTGTGATGTGATGAGTTTAGAAGTAAGAGTTAATAATAAA
    GTGGCACAACATGTATATGAAAATTTAGGATTTCAATATGGTGGTAAACGTAAAAATTAT
    TATGGTGAAGGTGAGGACGCAATGGTTATGTGGGTGAATTTAAATGACTAA
    60
    120
    180
    240
    300
    360
    411

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS10650 [old locus tag: SA1855 ]
  • symbol: SA_RS10650
  • description: ribosomal-protein-alanine N-acetyltransferase RimI
  • length: 136
  • theoretical pI: 4.43259
  • theoretical MW: 15902.7
  • GRAVY: -0.334559

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.128?  ExPASy
    Ribosomal-protein-alanine N-acetyltransferase Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine
  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 117)
    and 3 more
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 33.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 30.6)
    GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 16.3)
  • TheSEED: see SA1855
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 59.8)
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 48.8)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 48.6)
    and 6 more
    Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 27.4)
    FR47; FR47-like protein (PF08445; HMM-score: 26)
    Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 21.3)
    Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 14.5)
    Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 13.9)
    Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001487
    • TAT(Tat/SPI): 0.000143
    • LIPO(Sec/SPII): 0.000363
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPQVFDLERRSFNDSSWTIDAFYHEIEQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYASHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS10650&oldid=96389"