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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1957 [new locus tag: NWMN_RS11290 ]
  • pan locus tag?: SAUPAN005299000
  • symbol: rimJ
  • pan gene symbol?: rimI
  • synonym:
  • product: ribosomal-protein-alanine N-acetyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1957 [new locus tag: NWMN_RS11290 ]
  • symbol: rimJ
  • product: ribosomal-protein-alanine N-acetyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2167400..2167864
  • length: 465
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGGATCAACAGTCAAAAGAACAATTAAACATTAGAGAGATGACTAAAGAAGATGTGCCA
    CAAGTCTTTGATCTTGAGCGTCGGAGTTTCAATGACAGTTCTTGGACAATTGACGCATTT
    TATCATGAAATAGAGCAAAATAACTTTGCGAAATATTTTGTCTTAGAATTTGAACAACAA
    ATTATTGGTTATTTAGGTTTATGGATTGTTATCGATCAAGCTCAAATTACAACAGTTGCA
    ATCGATGATAATTATAGAGGCTATGGTTTAGGTCAAATGCTTTTAAAATACGGAATAAAC
    TATGCAAGTCACACTTGTGATGTGATGAGTTTAGAAGTAAGAGTTAATAATAAAGTGGCA
    CAACATGTATATGAAAATTTAGGATTTCAATATGGTGGTAAACGTAAAAATTATTATGGT
    GAAGGTGAGGACGCAATGGTTATGTGGGTGAATTTAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    465

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1957 [new locus tag: NWMN_RS11290 ]
  • symbol: RimJ
  • description: ribosomal-protein-alanine N-acetyltransferase
  • length: 154
  • theoretical pI: 4.37874
  • theoretical MW: 18076.1
  • GRAVY: -0.496104

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.128?  ExPASy
    Ribosomal-protein-alanine N-acetyltransferase Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine
  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 117.7)
    and 5 more
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 32.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 30.3)
    Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 17.1)
    putative beta-lysine N-acetyltransferase (TIGR03827; EC 2.3.1.-; HMM-score: 16.4)
    GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 15.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 59.6)
    Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 49.5)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 48.1)
    and 6 more
    Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 28.5)
    Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 26.3)
    Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 26)
    FR47; FR47-like protein (PF08445; HMM-score: 25.5)
    Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 14)
    Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005313
    • TAT(Tat/SPI): 0.000248
    • LIPO(Sec/SPII): 0.000693
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEIEQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYASHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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