PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• pan locus tag^?: SAUPAN005300000
• symbol: NWMN_1958
• pan gene symbol^?: tsaB
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• symbol: NWMN_1958
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2167837..2168526
• length: 690
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332356 NCBI
• RefSeq: YP_001332992 NCBI
• BioCyc:
• MicrobesOnline: 3707546 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAACAAATTAAGGAGGCAATTATCCATGAATTCGCTGCTCATTGATACATCGAATCAA
  CCATTATCAGTAGCATTGATGCAAAACGATGAAGTTTTAGCTGAAATAACTACGGATTCT
  AAACAAAATCATTCAGTGCAATTAATGCCTGCGATTAGTCAGCTATTTGAGCAGAGCAAA
  ATAGCTAAACAGCAATTAGATGCGATTATTGTTGCTGAAGGTCCTGGTTCATATACTGGC
  TTACGTATCGGTGTTACTGTTGCTAAAACATTAGCGTATGCGTTAGATGTTAAATTATAT
  GGTGTTTCATCATTAAAGGCATTGGCAGCAACAATCGATCATACTGATAAATTATTGGTT
  CCTGTGTTTGATGCAAGACGACAAGCAGTTTATACAGGCATGTTCCAGTGGCAAAATGGT
  CAATTAGTGACTATTTTAGAAGATCAATATATGTCGATAGAGGCATTGCGAGCTTTTTTA
  GAAGATTTAAACCAACCATTTATTTATATTGGGAAAGATACTGTAAAATTACAAGATGAA
  TTACAAGGTGAAGTGATTGCGCAGCTACCAAATGCATCAGTCATGTATCAATTGCTAGAT
  AAACCTTCAGACGTACATACATTTACTCCTAAATACCATAAATTAGCTGAGGCGGAACGA
  AATTGGATCAACAGTCAAAAGAACAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• symbol: NWMN_1958
• description: hypothetical protein
• length: 229
• theoretical pI: 5.67604
• theoretical MW: 25681.3
• GRAVY: -0.181659

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 178.9)
  and 3 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 39.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 34.7)
• TheSEED:  
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory YgjD and YeaZ  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  and 7 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  RNA Metabolism RNA processing and modification t6A synthesis in bacteria  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  YrdC-YciO  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  YrdC-YciO-Sua5 and associated protein families  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Bacterial RNA-metabolizing Zn-dependent hydrolases  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Conserved gene cluster associated with Met-tRNA formyltransferase  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
• PFAM:
  Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 108.4)
  and 1 more
  no clan defined DUF3126; Protein of unknown function (DUF3126) (PF11324; HMM-score: 12.3)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022393
  • TAT(Tat/SPI): 0.002476
  • LIPO(Sec/SPII): 0.001267
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222170 NCBI
• RefSeq: YP_001332992 NCBI
• UniProt: A0A0H3KHW1 UniProt

⊟Protein sequence

• MNKLRRQLSMNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGMFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKNN

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1956 < rimJ < NWMN_1958 < NWMN_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.