NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• pan locus tag^?: SAUPAN005300000
• symbol: NWMN_1958
• pan gene symbol^?: tsaB
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• symbol: NWMN_1958
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2167837..2168526
• length: 690
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332356 NCBI
• RefSeq: YP_001332992 NCBI
• BioCyc:
• MicrobesOnline: 3707546 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACAAATTAAGGAGGCAATTATCCATGAATTCGCTGCTCATTGATACATCGAATCAA
  CCATTATCAGTAGCATTGATGCAAAACGATGAAGTTTTAGCTGAAATAACTACGGATTCT
  AAACAAAATCATTCAGTGCAATTAATGCCTGCGATTAGTCAGCTATTTGAGCAGAGCAAA
  ATAGCTAAACAGCAATTAGATGCGATTATTGTTGCTGAAGGTCCTGGTTCATATACTGGC
  TTACGTATCGGTGTTACTGTTGCTAAAACATTAGCGTATGCGTTAGATGTTAAATTATAT
  GGTGTTTCATCATTAAAGGCATTGGCAGCAACAATCGATCATACTGATAAATTATTGGTT
  CCTGTGTTTGATGCAAGACGACAAGCAGTTTATACAGGCATGTTCCAGTGGCAAAATGGT
  CAATTAGTGACTATTTTAGAAGATCAATATATGTCGATAGAGGCATTGCGAGCTTTTTTA
  GAAGATTTAAACCAACCATTTATTTATATTGGGAAAGATACTGTAAAATTACAAGATGAA
  TTACAAGGTGAAGTGATTGCGCAGCTACCAAATGCATCAGTCATGTATCAATTGCTAGAT
  AAACCTTCAGACGTACATACATTTACTCCTAAATACCATAAATTAGCTGAGGCGGAACGA
  AATTGGATCAACAGTCAAAAGAACAATTAA
  60
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⊟Protein

⊟General

• locus tag: NWMN_1958 [new locus tag: NWMN_RS11295 ]
• symbol: NWMN_1958
• description: hypothetical protein
• length: 229
• theoretical pI: 5.67604
• theoretical MW: 25681.3
• GRAVY: -0.181659

⊟Function

• TIGRFAM:
  Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 178.9)
  and 3 more
  Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44)
  Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesmetallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 39.9)
  Genetic information processingDNA metabolismDNA replication, recombination, and repairuniversal archaeal protein Kae1 (TIGR03722; HMM-score: 34.7)
• TheSEED:  
  Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryYgjD and YeaZ TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  and 7 more
  Cell Wall and CapsuleCell Wall and Capsule - no subcategoryYjeE TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Protein MetabolismProtein biosynthesisRibosome biogenesis bacterial TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  RNA MetabolismRNA processing and modificationt6A synthesis in bacteria TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  YrdC-YciO TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  YrdC-YciO-Sua5 and associated protein families TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Bacterial RNA-metabolizing Zn-dependent hydrolases TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Conserved gene cluster associated with Met-tRNA formyltransferase TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
• PFAM:
  Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 108.4)
  and 1 more
  no clan definedDUF3126; Protein of unknown function (DUF3126) (PF11324; HMM-score: 12.3)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022393
  • TAT(Tat/SPI): 0.002476
  • LIPO(Sec/SPII): 0.001267
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222170 NCBI
• RefSeq: YP_001332992 NCBI
• UniProt: A0A0H3KHW1 UniProt

⊟Protein sequence

• MNKLRRQLSMNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGMFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKNN

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1956 < rimJ < NWMN_1958 < NWMN_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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