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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
- pan locus tag?: SAUPAN005300000
- symbol: SACOL2040
- pan gene symbol?: tsaB
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
- symbol: SACOL2040
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2099454..2100116
- length: 663
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236151 NCBI
- RefSeq: YP_186857 NCBI
- BioCyc:
- MicrobesOnline: 913513 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAATTCGCTGCTCATTGATACATCGAATCAACCATTATCAGTAGCATTGATGCAAAAC
GATGAAGTTTTAGCTGAAATAACTACGGATTCTAAACAAAATCATTCAGTGCAATTAATG
CCTGCGATTAGTCAGCTATTTGAGCAGAGCAAAATAGCTAAACAGCAATTAGATGCGATT
ATTGTTGCTGAAGGTCCTGGTTCATATACTGGCTTACGTATCGGTGTTACTGTTGCTAAA
ACATTAGCGTATGCGTTAGATGTTAAATTATATGGTGTTTCATCATTAAAGGCATTGGCA
GCAACAATCGATCATACTGATAAATTATTGGTTCCTGTGTTTGATGCAAGACGACAAGCA
GTTTATACAGGCATATTCCAGTGGCAAAATGGTCAATTAGTGACTATTTTAGAAGATCAA
TATATGTCGATAGAGGCATTGCGAGCTTTTTTAGAAGATTTAAACCAACCATTTATTTAT
ATTGGGAAAGATACTGTAAAATTACAAGATGAATTACAAGGTGAAGTGATTGCGCAGCTA
CCAAATGCATCAGTCATGTATCAATTGCTAGATAAACCTTCAGACGTACATACATTTACT
CCTAAATACCATAAATTAGCTGAGGCGGAACGAAATTGGATCAACAGTCAAAAGAACAAT
TAA60
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663
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
- symbol: SACOL2040
- description: hypothetical protein
- length: 220
- theoretical pI: 4.85595
- theoretical MW: 24535.8
- GRAVY: -0.126364
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 178.6)and 3 moreProtein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44.1)Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 40)DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 34.8)
- TheSEED: Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory YgjD and YeaZ TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNAand 7 moreCell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNAProtein Metabolism Protein biosynthesis Ribosome biogenesis bacterial TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNARNA Metabolism RNA processing and modification t6A synthesis in bacteria TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNAYrdC-YciO-Sua5 and associated protein families TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
- PFAM: Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 108.3)
⊟Structure, modifications & interactions[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008175
- TAT(Tat/SPI): 0.000694
- LIPO(Sec/SPII): 0.000638
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKNN
⊟Experimental data[edit | edit source]
- experimental localization: Cytoplasmic [1] [2]quantitative data / protein copy number per cell: 96 [3]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑
Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑
Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑
Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit | edit source]