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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
  • pan locus tag?: SAUPAN005300000
  • symbol: SACOL2040
  • pan gene symbol?: tsaB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
  • symbol: SACOL2040
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2099454..2100116
  • length: 663
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATTCGCTGCTCATTGATACATCGAATCAACCATTATCAGTAGCATTGATGCAAAAC
    GATGAAGTTTTAGCTGAAATAACTACGGATTCTAAACAAAATCATTCAGTGCAATTAATG
    CCTGCGATTAGTCAGCTATTTGAGCAGAGCAAAATAGCTAAACAGCAATTAGATGCGATT
    ATTGTTGCTGAAGGTCCTGGTTCATATACTGGCTTACGTATCGGTGTTACTGTTGCTAAA
    ACATTAGCGTATGCGTTAGATGTTAAATTATATGGTGTTTCATCATTAAAGGCATTGGCA
    GCAACAATCGATCATACTGATAAATTATTGGTTCCTGTGTTTGATGCAAGACGACAAGCA
    GTTTATACAGGCATATTCCAGTGGCAAAATGGTCAATTAGTGACTATTTTAGAAGATCAA
    TATATGTCGATAGAGGCATTGCGAGCTTTTTTAGAAGATTTAAACCAACCATTTATTTAT
    ATTGGGAAAGATACTGTAAAATTACAAGATGAATTACAAGGTGAAGTGATTGCGCAGCTA
    CCAAATGCATCAGTCATGTATCAATTGCTAGATAAACCTTCAGACGTACATACATTTACT
    CCTAAATACCATAAATTAGCTGAGGCGGAACGAAATTGGATCAACAGTCAAAAGAACAAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2040 [new locus tag: SACOL_RS10650 ]
  • symbol: SACOL2040
  • description: hypothetical protein
  • length: 220
  • theoretical pI: 4.85595
  • theoretical MW: 24535.8
  • GRAVY: -0.126364

Function[edit source | edit]

  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 178.6)
    and 3 more
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesmetallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 40)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairuniversal archaeal protein Kae1 (TIGR03722; HMM-score: 34.8)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryYgjD and YeaZ TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    and 7 more
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryYjeE TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    Protein MetabolismProtein biosynthesisRibosome biogenesis bacterial TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    RNA MetabolismRNA processing and modificationt6A synthesis in bacteria TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    YrdC-YciO TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    YrdC-YciO-Sua5 and associated protein families TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    Bacterial RNA-metabolizing Zn-dependent hydrolases TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
    Conserved gene cluster associated with Met-tRNA formyltransferase TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  • PFAM:
    Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 108.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008175
    • TAT(Tat/SPI): 0.000694
    • LIPO(Sec/SPII): 0.000638
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKNN

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1] [2]
    quantitative data / protein copy number per cell: 96 [3]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]