PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1856 [new locus tag: SA_RS10655 ]
• pan locus tag^?: SAUPAN005300000
• symbol: SA1856
• pan gene symbol^?: tsaB
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1856 [new locus tag: SA_RS10655 ]
• symbol: SA1856
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2095977..2096666
• length: 690
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124750 NCBI
• RefSeq: NP_375159 NCBI
• BioCyc: see SA_RS10655
• MicrobesOnline: 104185 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAACAAATTAAGGAGGCAATTATCCATGAATTCGCTGCTCATTGATACATCGAATCAA
  CCATTATCAGTAGCATTGATGCAAAACGATGAAGTTTTAGCTGAAATAACTACGGATTCT
  AAACAAAATCATTCAGTGCAATTAATGCCTGCGATTAGTCAGCTATTTGAGCAGAGCAAA
  ATAGCTAAACAGCAATTAGATGCGATTATTGTTGCTGAAGGTCCTGGTTCATATACTGGC
  TTACGTATCGGTGTTACTGTTGCTAAAACATTAGCGTATGCGTTAGATGTTAAATTATAT
  GGTGTTTCATCATTAAAGGCATTGGCAGCAACAATCGATCATACTGATAAATTATTGGTT
  CCTGTGTTTGATGCAAGACGACAAGCAGTTTATACAGGCATATTCCAGTGGCAAAATGGT
  CAATTAGTGACTATTTTAGAAGATCAATATATGTCGATAGAGGCATTGCGAGCTTTTTTA
  GAAGATTTAAACCAACCATTTATTTATATTGGGAAAGATACTATAAAATTACAAGATGAA
  TTACAAGGTGAAGTGATTGCGCAGCTACCAAATGCATCAGTCATGTATCAATTGATAGAT
  AAACCTACAGACGTACATACATTTACTCCTAAATACCATAAATTAGCTGAGGCGGAACGA
  AATTGGATCAACAGTCAAAAGAACAATTAA
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⊟Protein

⊟General

• locus tag: SA1856 [new locus tag: SA_RS10655 ]
• symbol: SA1856
• description: hypothetical protein
• length: 229
• theoretical pI: 5.67604
• theoretical MW: 25691.3
• GRAVY: -0.165502

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 177.9)
  and 3 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 39.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 34.7)
• TheSEED  :
  • TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory YgjD and YeaZ  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  and 4 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  RNA Metabolism RNA processing and modification YrdC-YciO  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
  RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA
• PFAM:
  Actin_ATPase (CL0108) TsaD; tRNA N6-adenosine threonylcarbamoyltransferase (PF00814; HMM-score: 119.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9974
  • Cytoplasmic Membrane Score: 0.0006
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0017
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022393
  • TAT(Tat/SPI): 0.002476
  • LIPO(Sec/SPII): 0.001267
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927626 NCBI
• RefSeq: NP_375159 NCBI
• UniProt: A0A0H3JR45 UniProt

⊟Protein sequence

• MNKLRRQLSMNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPTDVHTFTPKYHKLAEAERNWINSQKNN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1854 < SA1855 < SA1856 < SA1857

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications