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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • pan locus tag?: SAUPAN005301000
  • symbol: SA1857
  • pan gene symbol?: tsaE
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • symbol: SA1857
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2096620..2097081
  • length: 462
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGATAAAGATAAATAATTTAGATGAAATGAATCAATTTGCTATGTTTTTAGTTGAGCAA
    TTGAAAAGTGGTGATTTGATTTTACTTAACGGAGATTTAGGAGCAGGTAAAACAACGTTA
    ACGCAATTTATAGGAAAAGCTCTTGGTGTAAGACGTACGATTAATTCCCCGACATTTAAC
    ATCATTAAATCATATAGGGGTAAAAATTTAAAATTGCATCATATGGATTGTTATCGCTTA
    GAAGATTCTGATGAAGATTTAGGGTTTGATGAATTTTTCGAAGATCAGGCAATTACTGTT
    ATTGAATGGAGTCAATTTATAAAAGATTTACTTCCAGCGACGCATTTATCTATTAACATT
    TCAACAATATCTGAAAATACAAGACAAATTGAGTTGTTCGCGCAAGGAGAACATTATGAA
    CAAATTAAGGAGGCAATTATCCATGAATTCGCTGCTCATTGA
    60
    120
    180
    240
    300
    360
    420
    462

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • symbol: SA1857
  • description: hypothetical protein
  • length: 153
  • theoretical pI: 4.83329
  • theoretical MW: 17530.8
  • GRAVY: -0.21634

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 129.4)
    and 10 more
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 15.5)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 14.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 12.8)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.6)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12.2)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 12.1)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 11.8)
    Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 10.6)
  • TheSEED  :
    • tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    and 2 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  • PFAM:
    P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 124)
    and 12 more
    AAA_18; AAA domain (PF13238; HMM-score: 21.7)
    NACHT; NACHT domain (PF05729; HMM-score: 16.3)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 14.9)
    AAA_23; AAA domain (PF13476; HMM-score: 14.9)
    dNK; Deoxynucleoside kinase (PF01712; HMM-score: 14.6)
    ABC_tran; ABC transporter (PF00005; HMM-score: 13.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.1)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.7)
    AAA_22; AAA domain (PF13401; HMM-score: 12.6)
    AAA_33; AAA domain (PF13671; HMM-score: 12.5)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.1)
    TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009282
    • TAT(Tat/SPI): 0.002598
    • LIPO(Sec/SPII): 0.000705
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFIGKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIEWSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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