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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • pan locus tag?: SAUPAN005301000
  • symbol: SA1857
  • pan gene symbol?: tsaE
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • symbol: SA1857
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2096620..2097081
  • length: 462
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGATAAAGATAAATAATTTAGATGAAATGAATCAATTTGCTATGTTTTTAGTTGAGCAA
    TTGAAAAGTGGTGATTTGATTTTACTTAACGGAGATTTAGGAGCAGGTAAAACAACGTTA
    ACGCAATTTATAGGAAAAGCTCTTGGTGTAAGACGTACGATTAATTCCCCGACATTTAAC
    ATCATTAAATCATATAGGGGTAAAAATTTAAAATTGCATCATATGGATTGTTATCGCTTA
    GAAGATTCTGATGAAGATTTAGGGTTTGATGAATTTTTCGAAGATCAGGCAATTACTGTT
    ATTGAATGGAGTCAATTTATAAAAGATTTACTTCCAGCGACGCATTTATCTATTAACATT
    TCAACAATATCTGAAAATACAAGACAAATTGAGTTGTTCGCGCAAGGAGAACATTATGAA
    CAAATTAAGGAGGCAATTATCCATGAATTCGCTGCTCATTGA
    60
    120
    180
    240
    300
    360
    420
    462

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1857 [new locus tag: SA_RS10660 ]
  • symbol: SA1857
  • description: hypothetical protein
  • length: 153
  • theoretical pI: 4.83329
  • theoretical MW: 17530.8
  • GRAVY: -0.21634

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 129.4)
    and 10 more
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 15.5)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 14.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 12.8)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.6)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12.2)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 12.1)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 11.8)
    Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 10.6)
  • TheSEED:  
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory YjeE  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    and 3 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    RNA Metabolism RNA processing and modification t6A synthesis in bacteria  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
    YrdC-YciO-Sua5 and associated protein families  TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA
  • PFAM:
    P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 124)
    and 12 more
    AAA_18; AAA domain (PF13238; HMM-score: 21.7)
    NACHT; NACHT domain (PF05729; HMM-score: 16.3)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 14.9)
    AAA_23; AAA domain (PF13476; HMM-score: 14.9)
    dNK; Deoxynucleoside kinase (PF01712; HMM-score: 14.6)
    ABC_tran; ABC transporter (PF00005; HMM-score: 13.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.1)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.7)
    AAA_22; AAA domain (PF13401; HMM-score: 12.6)
    AAA_33; AAA domain (PF13671; HMM-score: 12.5)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 12.1)
    TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 12)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009282
    • TAT(Tat/SPI): 0.002598
    • LIPO(Sec/SPII): 0.000705
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFIGKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIEWSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]