PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1855 [new locus tag: SA_RS10650 ]
• pan locus tag^?: SAUPAN005299000
• symbol: SA1855
• pan gene symbol^?: rimI
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1855 [new locus tag: SA_RS10650 ]
• symbol: SA1855
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2095540..2096004
• length: 465
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124749 NCBI
• RefSeq: NP_375158 NCBI
• BioCyc: see SA_RS10650
• MicrobesOnline: 104184 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGGATCAACAGTCAAAAGAACAATTAAACATTAGAGAGATGACTAAAGAAGATGTGCCA
  CAAGTCTTTGATCTTGAGCGTCGGAGTTTCAATGACAGTTCTTGGACAATTGACGCATTT
  TATCATGAAATAGAGCAAAATAACTTTGCGAAATATTTTGTCTTAGAATTTGAACAACAA
  ATTATTGGTTATTTAGGTTTATGGATTGTTATCGATCAAGCTCAAATTACAACAGTTGCA
  ATCGATGATAATTATAGAGGCTATGGTTTAGGTCAAATGCTTTTAAAATACGGAATAAAC
  TATGCAAGTCACACTTGTGATGTGATGAGTTTAGAAGTAAGAGTTAATAATAAAGTGGCA
  CAACATGTATATGAAAATTTAGGATTTCAATATGGTGGTAAACGTAAAAATTATTATGGT
  GAAGGTGAGGACGCAATGGTTATGTGGGTGAATTTAAATGACTAA
  60
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  465
  

⊟Protein

⊟General

• locus tag: SA1855 [new locus tag: SA_RS10650 ]
• symbol: SA1855
• description: hypothetical protein
• length: 154
• theoretical pI: 4.37874
• theoretical MW: 18076.1
• GRAVY: -0.496104

⊟Function

• reaction:
  EC 2.3.1.128?  ExPASy
  Ribosomal-protein-alanine N-acetyltransferase Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine
• TIGRFAM:
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 117.7)
  and 5 more
  UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 32.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 30.3)
  Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 17.1)
  putative beta-lysine N-acetyltransferase (TIGR03827; EC 2.3.1.-; HMM-score: 16.4)
  GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 15.9)
• TheSEED  :
  • [Ribosomal protein S18]-alanine N-acetyltransferase (EC 2.3.1.266)
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)
  and 1 more
  RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-)
• PFAM:
  Acetyltrans (CL0257) Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 59.6)
  Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 49.5)
  Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 48.1)
  and 6 more
  Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 28.5)
  Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 26.3)
  Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 26)
  FR47; FR47-like protein (PF08445; HMM-score: 25.5)
  Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 14)
  Acetyltransf_CG; GCN5-related N-acetyl-transferase (PF14542; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005313
  • TAT(Tat/SPI): 0.000248
  • LIPO(Sec/SPII): 0.000693
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927625 NCBI
• RefSeq: NP_375158 NCBI
• UniProt: A0A0H3JM22 UniProt

⊟Protein sequence

• MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEIEQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYASHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1854 < SA1855 < SA1856 < SA1857

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications