PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1959 [new locus tag: NWMN_RS11300 ]
• pan locus tag^?: SAUPAN005301000
• symbol: NWMN_1959
• pan gene symbol^?: tsaE
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1959 [new locus tag: NWMN_RS11300 ]
• symbol: NWMN_1959
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2168480..2168974
• length: 495
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331210 NCBI
• RefSeq: YP_001332993 NCBI
• BioCyc:
• MicrobesOnline: 3707547 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGAATGAAAAGCATAATATAGGAGAATCAACATTGATAAAGATAAATAATTTAGATGAA
  ATGAATCAATTTGCTATATTTTTAGTTGAGCAATTGAAAAGTGGTGATTTGATTTTACTT
  AACGGAGATTTAGGAGCAGGTAAAACAACGTTAACGCAATTTATAGGAAAAGCTCTTGGT
  GTAAGACGTACGATTAATTCCCCGACATTTAACATCATTAAATCATATAGGGGTAAAAAT
  TTAAAATTGCATCATATGGATTGTTATCGCTTAGAAGATTCTGATGAAGATTTAGGATTT
  GATGAATTTTTCGAAGATCAGGCAATTACTGTTATTGAATGGAGTCAATTTATAAAAGAT
  TTACTTCCAGCGACGCATTTATCTATTAACATTTCAACAATATCTGAAAATACAAGACAA
  ATTGAGTTGTTCGCGCAAGGAGAACATTATGAACAAATTAAGGAGGCAATTATCCATGAA
  TTCGCTGCTCATTGA
  60
  120
  180
  240
  300
  360
  420
  480
  495
  

⊟Protein

⊟General

• locus tag: NWMN_1959 [new locus tag: NWMN_RS11300 ]
• symbol: NWMN_1959
• description: hypothetical protein
• length: 164
• theoretical pI: 4.87524
• theoretical MW: 18736.1
• GRAVY: -0.27561

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 128.2)
  and 9 more
  ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 15.4)
  Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 14.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 12.6)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.5)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.5)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 12)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 12)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 11.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 122.8)
  and 11 more
  AAA_18; AAA domain (PF13238; HMM-score: 21.5)
  NACHT; NACHT domain (PF05729; HMM-score: 16)
  RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 14.8)
  dNK; Deoxynucleoside kinase (PF01712; HMM-score: 14.5)
  AAA_23; AAA domain (PF13476; HMM-score: 14.3)
  ABC_tran; ABC transporter (PF00005; HMM-score: 13.2)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.9)
  AAA_33; AAA domain (PF13671; HMM-score: 12.3)
  TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 12)
  P-loop_NTPase (CL0023) Zeta_toxin; Zeta toxin (PF06414; HMM-score: 11.9)
  TIP49; TIP49 C-terminus (PF06068; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008217
  • TAT(Tat/SPI): 0.002061
  • LIPO(Sec/SPII): 0.00034
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222171 NCBI
• RefSeq: YP_001332993 NCBI
• UniProt: A0A0H3K9J5 UniProt

⊟Protein sequence

• MNEKHNIGESTLIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFIGKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIEWSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1956 < rimJ < NWMN_1958 < NWMN_1959

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications