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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS11295 [old locus tag: NWMN_1958 ]
- pan locus tag?: SAUPAN005300000
- symbol: NWMN_RS11295
- pan gene symbol?: tsaB
- synonym:
- product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS11295 [old locus tag: NWMN_1958 ]
- symbol: NWMN_RS11295
- product: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB
- replicon: chromosome
- strand: -
- coordinates: 2167837..2168499
- length: 663
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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541
601
661ATGAATTCGCTGCTCATTGATACATCGAATCAACCATTATCAGTAGCATTGATGCAAAAC
GATGAAGTTTTAGCTGAAATAACTACGGATTCTAAACAAAATCATTCAGTGCAATTAATG
CCTGCGATTAGTCAGCTATTTGAGCAGAGCAAAATAGCTAAACAGCAATTAGATGCGATT
ATTGTTGCTGAAGGTCCTGGTTCATATACTGGCTTACGTATCGGTGTTACTGTTGCTAAA
ACATTAGCGTATGCGTTAGATGTTAAATTATATGGTGTTTCATCATTAAAGGCATTGGCA
GCAACAATCGATCATACTGATAAATTATTGGTTCCTGTGTTTGATGCAAGACGACAAGCA
GTTTATACAGGCATGTTCCAGTGGCAAAATGGTCAATTAGTGACTATTTTAGAAGATCAA
TATATGTCGATAGAGGCATTGCGAGCTTTTTTAGAAGATTTAAACCAACCATTTATTTAT
ATTGGGAAAGATACTGTAAAATTACAAGATGAATTACAAGGTGAAGTGATTGCGCAGCTA
CCAAATGCATCAGTCATGTATCAATTGCTAGATAAACCTTCAGACGTACATACATTTACT
CCTAAATACCATAAATTAGCTGAGGCGGAACGAAATTGGATCAACAGTCAAAAGAACAAT
TAA60
120
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663
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS11295 [old locus tag: NWMN_1958 ]
- symbol: NWMN_RS11295
- description: tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB
- length: 220
- theoretical pI: 4.85595
- theoretical MW: 24553.9
- GRAVY: -0.138182
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 179.1)and 3 moreProtein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 44.1)Protein fate Degradation of proteins, peptides, and glycopeptides metallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 40)DNA metabolism DNA replication, recombination, and repair universal archaeal protein Kae1 (TIGR03722; HMM-score: 34.8)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 108.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.008175
- TAT(Tat/SPI): 0.000694
- LIPO(Sec/SPII): 0.000638
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAISQLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAATIDHTDKLLVPVFDARRQAVYTGMFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIYIGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQKNN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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