PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12305 [old locus tag: SA2143 ]
• pan locus tag^?: SAUPAN005853000
• symbol: SA_RS12305
• pan gene symbol^?: —
• synonym:
• product: HlyD family secretion protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12305 [old locus tag: SA2143 ]
• symbol: SA_RS12305
• product: HlyD family secretion protein
• replicon: chromosome
• strand: -
• coordinates: 2408561..2409208
• length: 648
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2408561..2409208) NCBI
• BioCyc: SA_RS12305 BioCyc
• MicrobesOnline: see SA2143

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAAAGATGATATTAATTAATGTCATTACGGTTGTTGTACTGTTAGCAATTGGGATT
  GCTGGCTTTTATTTCTGGAATAAAACAACAAGCTATGTAACAACTGATAATGCAAAAGTA
  AATGGCGATCAAATTAAAATAGCAAGTCCTGCATCTGGACAAATTAAATCACTTAATGTT
  AAACAAGGTGACAAACTCGATAAAGGTGACAAAGTAGCAACTGTTACTGTACAAGGTCAA
  GATGGCGAAACGAAAGATATGGATCTTAAAATGCCTCAAAAAGGTACAATTGCCAAATTA
  GATGGTATGGAAGGTTCAATGGTGCAAGCTGGTAACCCAATCGCTTATGCATACAATTTA
  GATGATTTATATGTAACAGCAAATATTGACGAAAAAGATATTAAAGATGTTGAAGTAGGT
  AAAGATGTTGATGTGACTATTGACGGTCAAAAAGCTTCTATTAAAGGTAAAGTTGATAGT
  ATTGGAAAAGCAACTGCTGCAAGCTTTTCATTAATGCCATCATCAAATAGTGATGGTAAC
  TACACTAAAGTTTCTCAAGTAATACCTGTTAAAATCACTCTAGAATCAGAACCGTCTAAA
  CAAGTTGTTCCTGGAATGAACGCTGAAGTAAAAATCCATAAAAATTAA
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⊟Protein

⊟General

• locus tag: SA_RS12305 [old locus tag: SA2143 ]
• symbol: SA_RS12305
• description: HlyD family secretion protein
• length: 215
• theoretical pI: 7.49286
• theoretical MW: 23012.2
• GRAVY: -0.231163

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Other efflux pump membrane protein (TIGR00998; HMM-score: 126.7)
  and 10 more
  Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 47.5)
  ABC exporter membrane fusion protein, DevB family (TIGR02971; HMM-score: 44)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 39.8)
  Metabolism Transport and binding proteins Other membrane fusion protein cluster 2 protein (TIGR00999; HMM-score: 32.4)
  Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 24.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 24.8)
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 22.7)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 17)
  Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 16.9)
  Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 14.3)
• TheSEED: see SA2143
• PFAM:
  Hybrid (CL0105) HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 64.6)
  HlyD_D23; Barrel-sandwich domain of CusB or HlyD membrane-fusion (PF16576; HMM-score: 56.1)
  and 6 more
  Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 37.9)
  Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 36.8)
  HlyD; HlyD membrane-fusion protein of T1SS (PF00529; HMM-score: 16.8)
  DUF2118; Uncharacterized protein conserved in archaea (DUF2118) (PF09891; HMM-score: 15)
  Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 14.6)
  DRMIP-like (CL0538) DUF4448; Protein of unknown function (DUF4448) (PF14610; HMM-score: 8.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.367511
  • TAT(Tat/SPI): 0.000603
  • LIPO(Sec/SPII): 0.070569
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446660904 NCBI
• RefSeq: WP_000738250 NCBI
• UniProt: see SA2143

⊟Protein sequence

• MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQIKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDGMEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDSIGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications