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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12370 [old locus tag: SA2154 ]
  • pan locus tag?: SAUPAN005873000
  • symbol: SA_RS12370
  • pan gene symbol?:
  • synonym:
  • product: DUF3021 domain-containing protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12370 [old locus tag: SA2154 ]
  • symbol: SA_RS12370
  • product: DUF3021 domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2419557..2420012
  • length: 456
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2419557..2420012) NCBI
  • BioCyc: SA_RS12370 BioCyc
  • MicrobesOnline: see SA2154

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAAATTTGAAAAACTCATTATTTATTAGCTTAATCATTGGTTTGTCCCTTTCACTG
    TTCTTTAGTATGTTATTTGCAGACGGCAAATATTATCCACTGAACCCACAATCAACTATT
    GGCATTTTGTATTATAATCATTTCACAGAAACTACCGTAATGTTGATTTCTATCATTTTA
    TGGTTACTCATAGGCGTCGTCTTTTTCCTTGGAGATTTTATCTTTAAATACACAGATTGG
    AGCATTACTAAAGCAACTATAATGCATTTCATAACAACGTATGTCGGATTCTTGCCTTTA
    GCAATGCTTGCCGGTTGGTTTCCACTAACAGTGCATTATCTAATCATATTCACAATTATC
    TTCATTGTGATTTATGTATTAATTTGGATAATTCAATTCTTTAAAAACAAGAACTACGTA
    GACACTATTAACGAGCAACTGAAACAATTAAAATGA
    60
    120
    180
    240
    300
    360
    420
    456

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS12370 [old locus tag: SA2154 ]
  • symbol: SA_RS12370
  • description: DUF3021 domain-containing protein
  • length: 151
  • theoretical pI: 9.47248
  • theoretical MW: 17704.2
  • GRAVY: 0.918543

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 9.4)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 9.4)
    Cellular processes Cellular processes Pathogenesis type VI secretion protein IcmF (TIGR03348; HMM-score: 9)
  • TheSEED: see SA2154
  • PFAM:
    no clan defined DUF3021; Protein of unknown function (DUF3021) (PF11457; HMM-score: 120.4)
    and 4 more
    DUF4271; Domain of unknown function (DUF4271) (PF14093; HMM-score: 14.2)
    DUF4381; Domain of unknown function (DUF4381) (PF14316; HMM-score: 12.4)
    AsmA-like (CL0401) AsmA; AsmA family (PF05170; HMM-score: 10.6)
    no clan defined Holin_SPP1; SPP1 phage holin (PF04688; HMM-score: 10)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.165681
    • TAT(Tat/SPI): 0.002333
    • LIPO(Sec/SPII): 0.143688
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILYYNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAMLAGWFPLTVHYLIIFTIIFIVIYVLIWIIQFFKNKNYVDTINEQLKQLK

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]