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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13470 [old locus tag: SA2351 ]
  • pan locus tag?: SAUPAN006228000
  • symbol: SA_RS13470
  • pan gene symbol?: crtP
  • synonym:
  • product: diapolycopene oxygenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13470 [old locus tag: SA2351 ]
  • symbol: SA_RS13470
  • product: diapolycopene oxygenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2641761..2643254
  • length: 1494
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2641761..2643254) NCBI
  • BioCyc: SA_RS13470 BioCyc
  • MicrobesOnline: see SA2351

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACTAAACATATCATCGTTATTGGTGGTGGCTTAGGTGGGATTTCTGCAGCAATTCGA
    ATGGCACAAAGTGGCTATTCGGTCTCATTATATGAACAAAATACTCATATAGGAGGCAAA
    GTGAATCGTCATGAATCAGATGGCTTTGGCTTTGATTTAGGTCCATCTATTTTAACGATG
    CCTTATATTTTTGAAAAATTATTCGAATATAGCAAGAAGCAAATGTCAGACTACGTTACA
    ATCAAGCGTTTGCCACATCAATGGCGTAGCTTTTTTCCAGATGGCACGACTATCGATTTG
    TATGAAGGTATTAAAGAAACAGGTCAGCATAATGCGATATTGTCGAAACAGGATATAGAG
    GAACTGCAAAATTATTTGAATTATACAAGACGAATCGATCGTATTACTGAAAAAGGGTAT
    TTTAACTATGGTTTAGATACACTATCTCAAATTATTAAATTTCATGGGCCATTAAATGCT
    CTTATTAATTATGATTATGTACATACTATGCAACAGGCCATAGACAAGCGTATCTCGAAT
    CCATACTTGCGACAAATGTTAGGCTATTTTATCAAATATGTAGGTTCTTCATCATACGAT
    GCGCCAGCTGTATTATCTATGTTATTCCATATGCAACAAGAGCAAGGCCTTTGGTATGTA
    GAAGGTGGAATCCATCATTTAGCCAATGCCTTGGAAAAGCTAGCGCGTGAAGAAGGTGTC
    ACAATTCATACAGGTGCACGTGTGGACAATATTAAAACATATCAAAGACGTGTGACGGGT
    GTCAGATTAGATACAGGTGAGTTTGTAAAGGCAGATTATATTATTTCAAATATGGAAGTC
    ATACCTACTTATAAATATTTAATTCACCTTGATACTCAACGATTAAACAAATTAGAGAGG
    GAATTTGAGCCGGCAAGCTCAGGATATGTGATGCATTTAGGTGTTGCTTGCCAATACCCG
    CAATTAGCACATCATAATTTCTTTTTTACGGAAAATGCTTATCTCAATTATCAACAAGTT
    TTTCATGAAAAGGTATTGCCAGATGATCCGACCATTTATCTAGTAAATACGAATAAAACT
    GATCACACACAAGCGCCAGTAGGTTATGAAAATATCAAAGTCTTACCACATATTCCATAT
    ATTCAAGATCAGCCTTTTACCACTGAAGATTATGCGAAGTTTAGGGATAAAATTTTGGAT
    AAATTAGAAAAAATGGGACTTACTGATTTAAGAAAACACATTATTTATGAAGATGTTTGG
    ACACCGGAGGATATTGAAAAAAATTATCGTTCTAATCGTGGTGCAATATATGGTGTTGTA
    GCAGATAAAAAGAAAAACAAAGGATTTAAATTTCCTAAAGAAAGTCAGTATTTTGAAAAC
    TTGTACTTTGTAGGTGGATCAGTAAATCCTGGTGGTGGCATGCCAATGGTTACATTAAGT
    GGGCAACAAGTCGCAGACAAAATAAACGCGCGAGAAGCGAAGAATAGGAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1494

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS13470 [old locus tag: SA2351 ]
  • symbol: SA_RS13470
  • description: diapolycopene oxygenase
  • length: 497
  • theoretical pI: 7.93809
  • theoretical MW: 57173.6
  • GRAVY: -0.485915

⊟Function[edit | edit source]

  • reaction:
    EC 1.14.99.44?  ExPASy
    Diapolycopene oxygenase 4,4'-diapolycopene + 4 AH2 + 4 O2 = 4,4'-diapolycopenedial + 4 A + 6 H2O
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 330.4)
    and 39 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other carotene isomerase (TIGR02730; EC 5.-.-.-; HMM-score: 97.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 87.5)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 39.1)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 36)
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 34.5)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 34.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 29.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin protoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 28.7)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 27.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 25)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin 3B synthase CobZ (TIGR02485; HMM-score: 22.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 22.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 19.6)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 18.8)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 17.8)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 17.8)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 17.6)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 16.6)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 15.2)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 15)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 15)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.6)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01811; EC 1.3.99.1; HMM-score: 14.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 14.3)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 13.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 13.9)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 13.8)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 13.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 13.1)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 12.6)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 12.2)
    Unknown function Enzymes of unknown specificity GMC family mycofactocin-associated oxidreductase (TIGR04542; EC 1.-.-.-; HMM-score: 12.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 12)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02731; EC 1.14.99.-; HMM-score: 11.7)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.4)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 11.4)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 11.1)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 10.6)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 10.4)
  • TheSEED: see SA2351
  • PFAM:
    NADP_Rossmann (CL0063) Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 88.9)
    and 15 more
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 52.3)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 43.1)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 43)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 35.3)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 35.2)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 34.5)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 33.1)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 31.1)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 17.2)
    Thi4; Thi4 family (PF01946; HMM-score: 13.3)
    Trp_halogenase; Tryptophan halogenase (PF04820; HMM-score: 13.2)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 12.7)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 11.5)
    MCRA; MCRA family (PF06100; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.199621
    • TAT(Tat/SPI): 0.016051
    • LIPO(Sec/SPII): 0.052487
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNTHIGGKVNRHESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYEGIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNALINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLWYVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIISNMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAYLNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYAKFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFKFPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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