From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0505 [new locus tag: NWMN_RS02940 ]
  • pan locus tag?: SAUPAN002315000
  • symbol: rpoC
  • pan gene symbol?: rpoC
  • synonym:
  • product: DNA-directed RNA polymerase subunit beta'

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0505 [new locus tag: NWMN_RS02940 ]
  • symbol: rpoC
  • product: DNA-directed RNA polymerase subunit beta'
  • replicon: chromosome
  • strand: +
  • coordinates: 579765..583388
  • length: 3624
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    TTGATTGATGTAAATAATTTCCATTATATGAAAATAGGATTGGCTTCACCTGAAAAAATC
    CGTTCTTGGTCTTTTGGTGAAGTTAAAAAACCTGAAACAATCAACTACCGTACATTAAAA
    CCTGAAAAAGATGGTCTATTCTGTGAAAGAATTTTCGGACCTACAAAAGACTGGGAATGT
    AGTTGTGGTAAATACAAACGTGTTCGCTACAAAGGCATGGTCTGTGACAGATGTGGAGTT
    GAAGTAACTAAATCTAAAGTACGTCGTGAAAGAATGGGTCACATTGAACTTGCTGCTCCA
    GTTTCTCACATTTGGTATTTCAAAGGTATACCAAGTCGTATGGGATTATTACTTGACATG
    TCACCAAGAGCATTAGAAGAAGTTATTTACTTTGCTTCTTATGTTGTTGTAGATCCAGGT
    CCAACTGGTTTAGAAAAGAAAACTTTATTATCTGAAGCTGAATTCAGAGATTATTATGAT
    AAATACCCAGGTCAATTCGTTGCAAAAATGGGTGCAGAAGGTATTAAAGATTTACTTGAA
    GAGATTGATCTTGACGAAGAACTTAAATTGTTACGCGATGAGTTGGAATCAGCTACTGGT
    CAAAGACTTACTCGTGCAATTAAACGTTTAGAAGTTGTTGAATCATTCCGTAATTCAGGT
    AACAAACCTTCATGGATGATTTTAGATGTACTTCCAATCATCCCACCAGAAATTCGTCCA
    ATGGTTCAATTAGATGGTGGACGATTTGCAACAAGTGACTTAAACGACTTATACCGTCGT
    GTAATTAATCGAAATAATCGTTTGAAACGTTTATTAGATTTAGGTGCACCTGGTATCATC
    GTTCAAAACGAAAAACGTATGTTACAAGAAGCCGTTGACGCTTTAATTGATAATGGTCGT
    CGTGGTCGTCCAGTTACTGGCCCAGGTAACCGTCCATTAAAATCTTTATCTCATATGTTA
    AAAGGTAAACAAGGTCGTTTCCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGA
    CGTTCAGTTATTGCAGTAGGTCCAAGCTTGAAAATGTACCAATGTGGTTTACCAAAAGAA
    ATGGCACTTGAACTATTTAAACCATTCGTAATGAAAGAATTAGTTCAACGTGAAATTGCA
    ACTAACATTAAAAATGCGAAGAGTAAAATCGAACGTATGGATGATGAAGTTTGGGACGTA
    TTGGAAGAAGTAATTAGAGAACATCCTGTATTACTTAACCGTGCACCAACACTTCATAGA
    CTTGGTATTCAAGCATTTGAACCAACTTTAGTTGAAGGTCGTGCGATTCGTCTACATCCA
    CTTGTAACAACAGCTTATAACGCTGACTTTGACGGTGACCAAATGGCGGTTCACGTTCCT
    TTATCAAAAGAGGCACAAGCTGAAGCAAGAATGTTGATGTTAGCAGCACAAAACATCTTG
    AACCCTAAAGATGGTAAACCTGTAGTTACACCATCACAAGATATGGTACTTGGTAACTAT
    TACCTTACTTTAGAAAGAAAAGATGCAGTAAATACAGGCGCAATCTTTAATAATACAAAT
    GAAGTATTAAAAGCATATGCAAATGGCTTTGTACATTTACACACTAGAATTGGTGTACAT
    GCAAGTTCGTTCAATAATCCAACATTTACTGAAGAACAAAACAAAAAGATTCTTGCTACG
    TCAGTAGGTAAAATTATATTCAATGAAATCATTCCAGATTCATTTGCTTATATTAATGAA
    CCTACGCAAGAAAACTTAGAAAGAAAGACACCAAACAGATATTTCATCGATCCTACAACT
    TTAGGTGAAGGTGGATTAAAAGAATACTTTGAAAATGAAGAATTAATTGAACCTTTCAAC
    AAAAAATTCTTAGGTAATATTATTGCAGAAGTATTCAACAGATTTAGCATCACTGATACA
    TCAATGATGTTAGACCGTATGAAAGACTTAGGATTCAAATTCTCATCTAAAGCTGGTATT
    ACAGTAGGTGTTGCTGATATCGTAGTATTACCTGATAAGCAACAAATACTTGATGAGCAT
    GAAAAATTAGTCGACAGAATTACAAAACAATTCAACCGTGGTTTAATCACTGAAGAAGAA
    AGATATAATGCAGTTGTTGAAATTTGGACAGATGCAAAAGATCAAATTCAAGGTGAATTG
    ATGCAATCACTTGATAAAACTAACCCAATCTTCATGATGAGTGATTCAGGTGCCCGTGGT
    AACGCATCTAACTTTACACAGTTAGCAGGTATGCGTGGATTGATGGCCGCACCATCTGGT
    AAGATTATCGAATTACCAATCACATCTTCATTCCGTGAAGGTTTAACAGTACTTGAATAC
    TTCATCTCAACTCACGGTGCACGTAAAGGTCTTGCCGATACAGCACTTAAAACAGCTGAC
    TCAGGATATCTTACTCGTCGTCTTGTTGACGTGGCACAAGATGTTATTGTTCGTGAAGAA
    GACTGTGGTACTGATAGAGGTTTATTAGTTTCTGATATTAAAGAAGGTACAGAAATGATT
    GAACCATTTATCGAACGTATTGAAGGTCGTTATTCTAAAGAAACAATTCGTCATCCTGAA
    ACTGATGAAATAATCATTCGTCCTGATGAATTAATTACACCTGAAATTGCTAAGAAAATT
    ACAGATGCTGGTATTGAACAAATGTATATTCGCTCAGCATTTACTTGTAACGCACGACAT
    GGTGTTTGTGAAAAATGTTACGGTAAAAACCTTGCTACTGGTGAAAAAGTTGAAGTTGGT
    GAAGCAGTTGGTACAATTGCAGCCCAATCTATCGGTGAACCAGGTACACAGCTTACAATG
    CGTACATTCCATACAGGTGGGGTAGCAGGTAGCGATATCACACAAGGTCTTCCTCGTATT
    CAAGAGATTTTCGAAGCACGTAACCCTAAAGGTCAAGCGGTAATTACGGAAATCGAAGGT
    GTCGTAGAAGATATTAAATTAGCAAAAGATAGACAACAAGAAATTGTTGTTAAAGGTGCT
    AATGAAACAAGATCATACCTTGCTTCAGGTACTTCAAGAATTATTGTAGAAATCGGTCAA
    CCAGTTCAACGTGGTGAAGTATTAACTGAAGGTTCTATTGAACCTAAGAATTACTTATCT
    GTTGCTGGATTAAACGCGACTGAAAGCTACTTATTAAAAGAAGTACAAAAAGTTTACCGT
    ATGCAAGGTGTAGAAATCGACGATAAACACGTTGAGGTTATGGTTCGACAAATGTTACGT
    AAAGTTAGAATTATCGAAGCAGGTGATACGAAGTTATTACCAGGTTCATTAGTTGATATT
    CATAACTTTACAGATGCAAATAGAGAAGCATTTAAACACCGTAAGCGTCCTGCAACAGCT
    AAACCAGTATTACTTGGTATTACTAAAGCATCACTTGAAACAGAAAGTTTCTTATCTGCA
    GCATCATTCCAAGAAACAACAAGAGTTCTTACAGATGCAGCAATTAAAGGTAAGCGTGAT
    GACTTATTAGGTCTTAAAGAAAACGTAATTATTGGTAAGTTAATTCCAGCTGGTACTGGT
    ATGAGACGTTATAGCGACGTAAAATACGAAAAAACAGCTAAACCAGTTGCAGAAGTTGAA
    TCTCAAACTGAAGTAACGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3624

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0505 [new locus tag: NWMN_RS02940 ]
  • symbol: RpoC
  • description: DNA-directed RNA polymerase subunit beta'
  • length: 1207
  • theoretical pI: 6.93506
  • theoretical MW: 135408
  • GRAVY: -0.353024

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.6?  ExPASy
    DNA-directed RNA polymerase Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
  • TIGRFAM:
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta' subunit (TIGR02386; EC 2.7.7.6; HMM-score: 1884.2)
    and 4 more
    DNA-directed RNA polymerase, gamma subunit (TIGR02387; EC 2.7.7.6; HMM-score: 850.1)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta subunit (TIGR02388; EC 2.7.7.6; HMM-score: 645.3)
    DNA-directed RNA polymerase subunit A' (TIGR02390; EC 2.7.7.6; HMM-score: 322.2)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, subunit A (TIGR02389; EC 2.7.7.6; HMM-score: 115)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined RNA_pol_Rpb1_1; RNA polymerase Rpb1, domain 1 (PF04997; HMM-score: 297.2)
    and 5 more
    RNA_pol_Rpb1_5; RNA polymerase Rpb1, domain 5 (PF04998; HMM-score: 233.4)
    RNA_pol_Rpb1_2; RNA polymerase Rpb1, domain 2 (PF00623; HMM-score: 140)
    RNA_pol_Rpb1_3; RNA polymerase Rpb1, domain 3 (PF04983; HMM-score: 117.5)
    RNA_pol_Rpb1_4; RNA polymerase Rpb1, domain 4 (PF05000; HMM-score: 54.7)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 17.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.052911
    • TAT(Tat/SPI): 0.002526
    • LIPO(Sec/SPII): 0.005436
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIDVNNFHYMKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFGPTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIPSRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAKMGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMILDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSVIAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVLEEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHVPLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFNNTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSFAYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFNRFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQFNRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLAGMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEIIIRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGVVEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYLSVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSLVDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAIKGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_2042(deoD)purine nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_1433(efp)elongation factor P  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_1937(groEL)chaperonin GroEL  [1] (data from MRSA252)
    NWMN_0005(gyrA)DNA gyrase A subunit  [1] (data from MRSA252)
    NWMN_1103(ileS)isoleucyl-tRNA synthetase  [1] (data from MRSA252)
    NWMN_0189(ispD)2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_0508(rpsG)30S ribosomal protein S7  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_1374hypothetical protein  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)
    NWMN_2201dehydrogenase family protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_0505&oldid=54359"