PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
• pan locus tag^?: SAUPAN003683000
• symbol: NWMN_1230
• pan gene symbol^?: cls1
• synonym:
• product: phospholipase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
• symbol: NWMN_1230
• product: phospholipase
• replicon: chromosome
• strand: +
• coordinates: 1353887..1355395
• length: 1509
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330732 NCBI
• RefSeq: YP_001332264 NCBI
• BioCyc:
• MicrobesOnline: 3706782 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  GTGCCTTTCTTTATAGGAGAAAAGACTATGCGATTTACATTTTCAAACGATTTAGGAACG
  TTATTTACTATTATTTTAGCCATTGGATTCATCATTAATTTAGTATTGGCTTTTATTATT
  ATCTTTTTAGAAAGAAATAGGCGTACAGCGAGTTCAACTTGGGCATGGCTATTTGTACTT
  TTTGTCTTACCATTGATTGGTTTTATTCTTTACTTGTTTTTTGGTAGAACCGTTTCGGCA
  CGCAAATTGAATAAAAACAATGGTAACGTGTTAACGGATTTCGATGGACTTTTAAAACAA
  CAAATAGAAAGCTTTGATAAAGGTAATTATGGTACTGATAACAAACAAGTTCAAAAACAT
  CATGATTTAGTACGTATGCTTTTGATGGATCAAGATGGTTTTTTAACTGAAAATAATAAA
  GTTGATCATTTCATTGATGGAAATGATTTATATGATCAAGTTTTAAAAGATATTAAAAAT
  GCAAAAGAATATATCCATTTAGAGTACTATACTTTCGCTTTAGATGGTTTAGGTAAAAGA
  ATTTTACATGCTTTAGAAGAAAAATTGAAACAAGGTCTAGAAGTAAAAATATTATATGAT
  GATGTTGGATCTAAAAATGTTAAGATGGCAAATTTTGATCATTTTAAATCGTTAGGTGGA
  GAAGTTGAAGCATTTTTTGCTTCAAAATTACCGTTATTGAATTTCAGAATGAATAATAGA
  AATCATAGAAAAATCATCGTAATCGATGGTCAACTAGGTTATGTCGGAGGATTTAACATT
  GGTGATGAATATCTAGGATTAGGAAAATTAGGATATTGGAGAGATACGCATTTACGTATA
  CAAGGGGATGCGGTTGATGCACTGCAGTTGCGATTTATTTTAGACTGGAATTCGCAAGCG
  CACCGTCCACAATTTGAATATGATGTTAAGTATTTCCCTAAAAAGAACGGACCATTGGGC
  AATTCACCAATTCAAATAGCTGCAAGTGGCCCGGCTAGTGACTGGCATCAAATTGAATAC
  GGTTATACAAAAATGATTATGAGTGCAAAGAAATCTGTATATTTACAATCACCATATTTC
  ATTCCGGATAATTCATATATAAATGCCATTAAAATTGCTGCTAAATCAGGTGTAGATGTA
  CATTTAATGATTCCATGTAAGCCAGATCATCCATTAGTATATTGGGCGACATTTTCAAAT
  GCCTCTGACTTATTATCAAGTGGTGTTAAAATTTATACGTATGAAAATGGATTTATACAT
  TCTAAAATGTGCTTAATTGATGATGAAATCGTATCAGTGGGCACAGCAAATATGGACTTT
  AGAAGTTTTGAATTAAATTTTGAAGTAAATGCCTTTGTATATGATGAAAATCTTGCTAAA
  GATTTAAGGGTGGCTTATGAACATGATATTACAAAATCAAAACAACTAACCAAAGAATCA
  TATGCCAATAGACCGCTGTCTGTTAAATTCAAAGAATCGTTAGCAAAATTAGTTTCGCCA
  ATTTTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1509
  

⊟Protein

⊟General

• locus tag: NWMN_1230 [new locus tag: NWMN_RS06950 ]
• symbol: NWMN_1230
• description: phospholipase
• length: 502
• theoretical pI: 7.81952
• theoretical MW: 57416.6
• GRAVY: -0.136653

⊟Function

• reaction:
  EC 2.7.8.-?  ExPASy
  2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
• TIGRFAM:
  cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 822.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 145.6)
  and 7 more
  PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 51)
  no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 45)
  CopD_like (CL0430) UPF0093; Uncharacterised protein family (UPF0093) (PF03653; HMM-score: 15.5)
  no clan defined Pox_A14; Poxvirus virion envelope protein A14 (PF05767; HMM-score: 14.7)
  DUF3611; Protein of unknown function (DUF3611) (PF12263; HMM-score: 13.6)
  MFS (CL0015) MFS_Mycoplasma; Mycoplasma MFS transporter (PF07672; HMM-score: 13.2)
  no clan defined DUF2207; Predicted membrane protein (DUF2207) (PF09972; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000898
  • TAT(Tat/SPI): 0.000089
  • LIPO(Sec/SPII): 0.008617
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 151221442 NCBI
• RefSeq: YP_001332264 NCBI
• UniProt: A0A0H3K8D4 UniProt

⊟Protein sequence

• MPFFIGEKTMRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASSTWAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYGTDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEYYTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLAKDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications