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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0640 [new locus tag: SACOL_RS03305 ]
  • pan locus tag?: SAUPAN002375000
  • symbol: SACOL0640
  • pan gene symbol?: merA
  • synonym:
  • product: pyridine nucleotide-disulfide oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0640 [new locus tag: SACOL_RS03305 ]
  • symbol: SACOL0640
  • product: pyridine nucleotide-disulfide oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 671727..673049
  • length: 1323
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAACATATGATTTAATTGTAATAGGATTTGGGAAAGCTGGTAAAACTTTAGCGAAA
    TACGCTGCATCAACAGGTCAACACGTCGCAGTTATCGAACAATCTCCGAAAATGTATGGA
    GGCACTTGTATAAACATAGGATGTATACCTTCGAAGACACTTGTACATGATGGATTAGAA
    GGCAAATCTTTTGAAGCAAGTTATAACCGTAAAAACGATGTTGTCAATGCGCTAAACAAT
    AAAAATTACCACTTATTAGCAGACGATAACAACATTGATGTACTGGATTTTAAAGCGCAG
    TTTAAATCTAATACTGAAGTGAATTTATTAGATCAACATGACGATATCGTCGATAGTATT
    ACTGCACCTCATATCATTATTAATACCGGTGCTACCTCTGTCATTCCTAACATTAAAGGC
    CTTGATCAAGCTAAACACGTCTTCGATTCGACAGGTTTATTAAACATTAGCTATCAACCT
    AAGCACCTCGTTATTGTAGGTGGCGGTTATATCGCCTTAGAATTTGCTTCAATGTTTGCG
    AATTTAGGTAGTAAGGTAACAGTATTAGAACGTGGCGAATCATTTATGCCACGCGAAGAT
    CAAGATGTCGTTGCATATGGTATTACTGACTTAGAAAATAAAGGCATTGCATTGCATACA
    AATGTTGAAACGACTGAATTGTCATCTGACAATCATCATACAACAGTCCATACCAACGTT
    GATAACTTTGAGGCTGATGCAGTACTTTTGGCTATCGGGCGCAAACCGAATACGGATTTA
    GCTTTAGAAAATACTGATATCGAATTAGGCGACAGAGGCGAAATTAAAGTCAATGCTCAT
    CTTCAAACAACTGTGCCGCATATTTATGCTGCAGGTGATGTTAAAGGCGGACTTCAATTT
    ACGTATATATCTTTAGATGATTATCGAATTATCAAATCAGCGTTATATGGTAATCAGTCA
    CGTACGACTGACAATAGAGGCAGCGTGCCTTATACAGTATTTATAGATCCACCATTATCA
    CGTGTTGGATTAACTAGTAAAGAAGCTGCCGCTCAACATTATGATTACACTGAACATCAA
    CTTTTAGTAAGTGCTATACCTCGTCATAAAATTAACAATGATCCAAGAGGTTTATTTAAA
    GTAGTCATTAATAATGAAAATAATATGATTTTAGGTGCTACATTATATGGTAAGCAATCT
    GAAGAATTAATTAATATAATTAAACTTGCGATTGATCAAAACATTCCATATACCGTATTA
    CGAGATAATATTTATACGCATCCTACGATGGCCGAATCATTTAATGATTTATTTAATTTC
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1323

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0640 [new locus tag: SACOL_RS03305 ]
  • symbol: SACOL0640
  • description: pyridine nucleotide-disulfide oxidoreductase
  • length: 440
  • theoretical pI: 5.65506
  • theoretical MW: 48473.2
  • GRAVY: -0.234318

⊟Function[edit | edit source]

  • TIGRFAM:
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 319.9)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 307.9)
    and 39 more
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 241.3)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 203.6)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 203.3)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 159)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 151.6)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 120)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 89.1)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 76.5)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 60.4)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 60.4)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 57.3)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 49.8)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 48.2)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 47.4)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 39)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 37.9)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 36.4)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 33.4)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 31.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 30.7)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 28.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 24.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 23.3)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 20.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 19.5)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 17.9)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 17.5)
    Genetic information processing Protein fate Protein folding and stabilization xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 17)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 17)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 16.5)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 16)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 15.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 14.5)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.4)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 14.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 13.9)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 13.6)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 13.5)
    lycopene cyclase family protein (TIGR01790; HMM-score: 12.3)
  • TheSEED  :
    • Mercuric ion reductase (EC 1.16.1.1)
    • PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
    • Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
    and 3 more
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  Mercuric ion reductase (EC 1.16.1.1)
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercury resistance operon  Mercuric ion reductase (EC 1.16.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 203.3)
    and 21 more
    Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 81.9)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 80.7)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 53.3)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 33.7)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 29.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 27.5)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 27.1)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 25.7)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 25.6)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 23.7)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 23.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 23.1)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 21.7)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.4)
    Thi4; Thi4 family (PF01946; HMM-score: 20.9)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 19.9)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 19.4)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.4)
    no clan defined DUF2704; Protein of unknown function (DUF2704) (PF10866; HMM-score: 15.8)
    NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 15.4)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.166332
    • TAT(Tat/SPI): 0.013324
    • LIPO(Sec/SPII): 0.030263
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCINIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFKSNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQPKHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIALHTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIKVNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVFIDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILGATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 74 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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