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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0641 [new locus tag: SACOL_RS03310 ]
  • pan locus tag?: SAUPAN002438000
  • symbol: SACOL0641
  • pan gene symbol?: hypR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0641 [new locus tag: SACOL_RS03310 ]
  • symbol: SACOL0641
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 673036..673476
  • length: 441
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGAATTTAGAATTTAACATTGCCGTGCATGTATTAGCTTTTTTAACTAAGCATCATTCA
    GAAAAATTCAATAGTAGTTCATTAGCAGAATTAACTTGTTTAAATCCTGTTCAATTACGA
    CGCGTGACGACTCAACTTGTCGATTTAAAAATGATTGACACAATACGAGGTAAAGATGGC
    GGTTATTTAGCAAATGATCAAAGTGCTGATGTCTCTCTAGCAACATTATATAAACATTTT
    GTCTTAGAGAAAGAACACCACACACGTCTATTTACTGGCGACGAAGGCAGTCACTGTCAA
    ATTGCTCGTAATATTGCAACTACCATGTCACATTATCAGCAAGACGAACAGAATATCATT
    ATTAATTTTTATAATGGAAAAACAATCAAAGATGTCATTGAAGACATTCAAAAGGAGGAT
    TTATGTCATGAAAACATATGA
    60
    120
    180
    240
    300
    360
    420
    441

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0641 [new locus tag: SACOL_RS03310 ]
  • symbol: SACOL0641
  • description: hypothetical protein
  • length: 146
  • theoretical pI: 6.22139
  • theoretical MW: 16698.8
  • GRAVY: -0.367808

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 38.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly SUF system regulator (TIGR02944; HMM-score: 32.7)
    Signal transduction Regulatory functions DNA interactions FeS assembly SUF system regulator (TIGR02944; HMM-score: 32.7)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster assembly transcription factor IscR (TIGR02010; HMM-score: 20.8)
    Signal transduction Regulatory functions DNA interactions iron-sulfur cluster assembly transcription factor IscR (TIGR02010; HMM-score: 20.8)
  • TheSEED  :
    • hypothetical fig|282458.1.peg.572 homolog
  • PFAM:
    HTH (CL0123) Rrf2; Transcriptional regulator (PF02082; HMM-score: 54.6)
    and 3 more
    TFIIE_alpha; TFIIE alpha subunit (PF02002; HMM-score: 15.1)
    Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 14.5)
    HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019128
    • TAT(Tat/SPI): 0.001531
    • LIPO(Sec/SPII): 0.002791
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTTQLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIARNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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