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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2352 [new locus tag: SACOL_RS12350 ]
  • pan locus tag?: SAUPAN005861000
  • symbol: tcaA
  • pan gene symbol?: tcaA
  • synonym:
  • product: tcaA protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2352 [new locus tag: SACOL_RS12350 ]
  • symbol: tcaA
  • product: tcaA protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2411156..2412538
  • length: 1383
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAATCTTGCCCGAAGTGCGGACAACAAGCACAAGATGATGTACAGATATGTACACAA
    TGTGGACATAAATTTGATAGTCGTCAAGCATTTTATAGAAAGTCAACCGATGAAGATATA
    CAAACTAATAACATCAAGATGAGAAAAATGGTGCCTTGGGCAATTGGTTTTTTCATTTTA
    ATCTTAATTATTATATTGTTCTTTTTACTAAGAAACTTCAATTCACCTGAAGCGCAAACT
    AAAATATTAGTGAATGCGATTGAGAATAATGATAAACAAAAAGTAGCAACATTATTAAGT
    ACTAAAGATAATAAAGTAGATTCTGAAGAAGCAAAAGTATACATTAACTATATCAAAGAT
    GAAGTTGGGCTTAAGCAATTTGTCAGCGACCTTAAAAATACGGTACATAAATTGAATAAG
    AGTAAGACCAGCGTAGCTTCTTATATTCAAACCAGATCTGGTCAAAATATATTACGTGTA
    AGTAAAAATGGCACACGTTATATCTTTTTCGATAATATGAGCTTTACTGCACCTACCAAG
    CAACCAATTGTTAAACCGAAAGAAAAAACAAAATATGAGTTTAAATCTGGTGGTAAGAAA
    AAGATGGTTATAGCTGAAGCAAATAAAGTGACGCCAATAGGTAATTTTATACCGGGGACA
    TATAGAATTCCAGCTATGAAATCAACTGAGAACGGTGATTTTGCAGGCCATTTAAAATTT
    GATTTTAGACAAAGTAATTCTGAAACGGTAGATGTTACTGAAGATTTTGAAGAAGCAAAT
    ATATCTGTAACTTTAAAAGGCGATACAAAATTAAATGATAGTTCTAAAAAAGTAACTATA
    AATGACCATGAAATGGCATTTTCAAGTTCCAAAACGTATGGTCCATATCCACAAAATAAA
    GATATTACCATTTCAGCTTCAGGTAAAGCGAAAGATAAAACATTTACAACACAGACGAAG
    ACGATTAAAGCTAGCGATTTAAAATACAATACAGAGATAACTTTGAATTTTGACAGTGAA
    GATATCGAAGACTATGTTGAAAAGAAAGAAAAAGAAGAAAACAGCTTGAAGAACAAATTG
    ATAGAATTCTTTGCTGGATATTCTTTAGCGAATAATGCTGCGTTTAATCAGTCGGATTTT
    GATTTTGTATCATCATATATAAAAAAAGGATCATCTTTTTATGATGATGTAAAGAAGCGT
    GTATCTAAAGGAAGTTTAATGATGATTAGTTCACCACAAATTATAGATGCTGAAAAACAT
    GGTGATAAGATTACCGCAACCGTAAGATTAATAAATGAAAACGGTAAGCAAGTAGATAAA
    GAATATGAGCTTGAACAAGGCTCGCAAGACCGCTTGCAATTAATCAAGACATCAGAAAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1383

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2352 [new locus tag: SACOL_RS12350 ]
  • symbol: TcaA
  • description: tcaA protein
  • length: 460
  • theoretical pI: 9.47124
  • theoretical MW: 52147.8
  • GRAVY: -0.655

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 13.8)
    and 1 more
    Cys-rich peptide, TIGR04165 family (TIGR04165; HMM-score: 5.7)
  • TheSEED  :
    • Teicoplanin resistance associated membrane protein TcaA
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Teicoplanin-resistance in Staphylococcus  Teicoplanin resistance associated membrane protein TcaA
  • PFAM:
    Zn_Beta_Ribbon (CL0167) zf-ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 28.3)
    zinc_ribbon_2; zinc-ribbon domain (PF13240; HMM-score: 25.3)
    and 13 more
    UPF0547; Uncharacterised protein family UPF0547 (PF10571; HMM-score: 19.3)
    DZR; Double zinc ribbon (PF12773; HMM-score: 18.4)
    Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 14.8)
    no clan defined DUF2950; Protein of unknown function (DUF2950) (PF11453; HMM-score: 14.1)
    ClpP_crotonase (CL0127) CLP_protease; Clp protease (PF00574; HMM-score: 14)
    MBB (CL0193) OprB; Carbohydrate-selective porin, OprB family (PF04966; HMM-score: 13.8)
    no clan defined DUF1461; Protein of unknown function (DUF1461) (PF07314; HMM-score: 11.7)
    RING (CL0229) Prok-RING_1; Prokaryotic RING finger family 1 (PF14446; HMM-score: 11.3)
    Zn_Beta_Ribbon (CL0167) zinc_ribbon_15; zinc-ribbon family (PF17032; HMM-score: 11)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 10.3)
    Transporter (CL0375) Clc-like; Clc-like (PF07062; HMM-score: 9.6)
    Zn_Beta_Ribbon (CL0167) OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 9.3)
    no clan defined YhfH; YhfH-like protein (PF14149; HMM-score: 9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008213
    • TAT(Tat/SPI): 0.000171
    • LIPO(Sec/SPII): 0.030623
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSCPKCGQQAQDDVQICTQCGHKFDSRQAFYRKSTDEDIQTNNIKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTKDNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRVSKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIPGTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSKKVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEITLNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSSFYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQDRLQLIKTSEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

M Brandenberger, M Tschierske, P Giachino, A Wada, B Berger-Bächi
Inactivation of a novel three-cistronic operon tcaR-tcaA-tcaB increases teicoplanin resistance in Staphylococcus aureus.
Biochim Biophys Acta: 2000, 1523(2-3);135-9
[PubMed:11042376] [WorldCat.org] [DOI] (P p)Hideki Maki, Nadine McCallum, Markus Bischoff, Akihito Wada, Brigitte Berger-Bächi
tcaA inactivation increases glycopeptide resistance in Staphylococcus aureus.
Antimicrob Agents Chemother: 2004, 48(6);1953-9
[PubMed:15155184] [WorldCat.org] [DOI] (P p)N McCallum, G Spehar, M Bischoff, B Berger-Bächi
Strain dependence of the cell wall-damage induced stimulon in Staphylococcus aureus.
Biochim Biophys Acta: 2006, 1760(10);1475-81
[PubMed:16891058] [WorldCat.org] [DOI] (P p)Nadine McCallum, Ann Karen C Brassinga, Costi D Sifri, Brigitte Berger-Bächi
Functional characterization of TcaA: minimal requirement for teicoplanin susceptibility and role in Caenorhabditis elegans virulence.
Antimicrob Agents Chemother: 2007, 51(11);3836-43
[PubMed:17709474] [WorldCat.org] [DOI] (P p)

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