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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS11500 [old locus tag: SA1998 ]
  • pan locus tag?: SAUPAN005594000
  • symbol: SA_RS11500
  • pan gene symbol?: lacR
  • synonym:
  • product: DeoR/GlpR transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS11500 [old locus tag: SA1998 ]
  • symbol: SA_RS11500
  • product: DeoR/GlpR transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 2269393..2270184
  • length: 792
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2269393..2270184) NCBI
  • BioCyc: G1G21-2328 BioCyc
  • MicrobesOnline: see SA1998

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACCCATCGCCTAAATAAAGGAGGCAATTTTACATTGAATAAACATGAACGTTTGGAC
    GAAATTGCTAAACTAGTGAATAAAAAGGGCACGATAAGAACGAATGAAATCGTCGAAGGT
    TTAAATGTGTCTGATATGACAGTTCGAAGAGATTTAATTGAGTTGGAAAATAAGGGCATT
    TTAACGAAGATTCATGGTGGTGCACGCAGTAATTCAACATTTCAGTATAAAGAAATATCA
    CATAAAGAAAAACATACTCGTCAAATTGCTGAAAAAAGATATATAGCTAGGAAAGCTGCA
    TCATTAATTGAAGATGGGGATACTTTGTTTTTCGGACCGGGAACAACAGTGGAACTATTA
    GCAGAAGAAGTCAATCATCATACGCTCACAATTATTACGAATTGTTTGCCGGTGTATAAA
    ATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTCTATTTAATTGGTGGTGAAATGCGC
    CATATTACAGAAGCATTTGTAGGTGAAATGGCGAATGCCATGTTGGAAAAACTAAGATTT
    AGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAAGGTGCTGTGATGACATCTACATTA
    GATGAAGCCTATACGCAACAATTGGCACTAAGTAATTCAATTGAAAAATACTTGTTAATC
    GATCATACGAAAGTTGGCAAAGAAGATTTTACATCATTTTGTCAGTTAAACGAATTGACT
    GCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTAGAAACGATTAAAACATATATTGAA
    GTAGTCGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS11500 [old locus tag: SA1998 ]
  • symbol: SA_RS11500
  • description: DeoR/GlpR transcriptional regulator
  • length: 263
  • theoretical pI: 6.68373
  • theoretical MW: 29932.1
  • GRAVY: -0.337643

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 24.6)
    and 5 more
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 15.7)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 15.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
    Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.7)
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 14.1)
  • TheSEED: see SA1998
  • PFAM:
    ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 162.2)
    and 8 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 81)
    HTH_11; HTH domain (PF08279; HMM-score: 27.4)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.2)
    FaeA; FaeA-like protein (PF04703; HMM-score: 17.5)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 16.2)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15)
    Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 13.1)
    PaaX; PaaX-like protein (PF07848; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by LacR, TF important in Lactose utilization: see SA1998

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.031751
    • TAT(Tat/SPI): 0.000485
    • LIPO(Sec/SPII): 0.000351
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRYIARKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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