NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1862 [new locus tag: SACOL_RS09550 ]
- pan locus tag?: SAUPAN004556000
- symbol: hsdM2
- pan gene symbol?: hsdM2
- synonym:
- product: type I restriction-modification system, M subunit
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1862 [new locus tag: SACOL_RS09550 ]
- symbol: hsdM2
- product: type I restriction-modification system, M subunit
- replicon: chromosome
- strand: -
- coordinates: 1914658..1916214
- length: 1557
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237807 NCBI
- RefSeq: YP_186690 NCBI
- BioCyc: see SACOL_RS09550
- MicrobesOnline: 913304 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCG
ATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTAGGC
TTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTGTCA
GGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTAAAA
GCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCGATG
ATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGCAAA
GTTGAAACATCTACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGCGAT
ATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATCTCT
AAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGATATG
TTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCA
GGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGT
AAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTACGT
GTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACATAC
AACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATCCGT
AATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCG
AACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGATTC
AGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACACATG
GTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTATTC
CGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAA
GCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATTTTA
GTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAATGAT
TTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATAGAC
ACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAGATT
GCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAAGCA
CCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCAGAA
ATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1862 [new locus tag: SACOL_RS09550 ]
- symbol: HsdM2
- description: type I restriction-modification system, M subunit
- length: 518
- theoretical pI: 4.38082
- theoretical MW: 59443.2
- GRAVY: -0.491506
⊟Function[edit | edit source]
- reaction: EC 2.1.1.-? ExPASy
- ⊞TIGRFAM: DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.6)and 5 more
- ⊞TheSEED :
- Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)and 1 more - ⊞PFAM: NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372.3)and 7 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)