From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01933
  • pan locus tag?: SAUPAN004556000
  • symbol: SAOUHSC_01933
  • pan gene symbol?: hsdM2
  • synonym:
  • product: type I restriction-modification system subunit M

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01933
  • symbol: SAOUHSC_01933
  • product: type I restriction-modification system subunit M
  • replicon: chromosome
  • strand: -
  • coordinates: 1840527..1842266
  • length: 1740
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGC
    GCCACTAAAACTCAAAATTTCCACCACCAACATCCAAATTATCAACATCGCAACATAACC
    AAATGTTATAATAAATCTATTACACAAAGAGATAAATTACTTATGCAAAGGCGGAGGAAT
    CACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
    TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTA
    GGCTTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTG
    TCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTA
    AAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
    ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGC
    AAAGTTGAAACATCTACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGC
    GATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATC
    TCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
    ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAA
    GCAGGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGAC
    GGTAAAGATAAATTACGTCACGTGTACGACCCAACATGTGGTTCCGGTTCATTGTTGTTA
    CGTGTTGGTAAAGAAACGCAAGTGTATCGTTATTTCGGACAAGAACGTAACAATACTACA
    TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGAGATC
    CGTAATGATGACACATTGGAAAATCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATT
    GCGAACCCACCATACAGTGCGAAATGGACAGCAGATTCAAAATTTGAAAATGATGAACGA
    TTCAGTGGTTACGGCAAGCTTGCGCCAAAATCCAAAGCAGACTTTGCCTTTATTCAACAC
    ATGGTACATTACCTAGACGATGAAGGTACCATGGCAGTCGTACTCCCACATGGTGTATTA
    TTCCGTGGTGCCGCAGAAGGTGTGATTCGTCGCTATTTAATAGAAGAAAAGAACTACTTA
    GAAGCCGTGATTGGGTTACCAGCCAATATTTTCTATGGGACAAGTATTCCAACATGTATT
    TTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
    GATTTTGAAAAAGGAAAAAATCAAAACCATTTAAGCGATGCCCAAGTCGAACGTATTATA
    GACACATATAAGCGTAAAGCAACGATTGATAAATATAGCTACAGCGCGACATTACAAGAG
    ATCGCCGATAACGATTACAACTTAAACATACCGCGATATGTTGATACATTCGAAGAAGAA
    GCACCAATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGACAAAGAAATCGCA
    GAAATTGAACAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01933
  • symbol: SAOUHSC_01933
  • description: type I restriction-modification system subunit M
  • length: 579
  • theoretical pI: 4.80289
  • theoretical MW: 66903.7
  • GRAVY: -0.553368

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467)
    and 5 more
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 37.2)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 36.5)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 16.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 14.3)
  • TheSEED  :
    • Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    and 1 more
    DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 370.7)
    and 7 more
    no clan defined HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 25)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.7)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.4)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.2)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.060796
    • TAT(Tat/SPI): 0.001007
    • LIPO(Sec/SPII): 0.013465
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_01933&oldid=101352"