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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001802
  • pan locus tag?: SAUPAN004556000
  • symbol: JSNZ_001802
  • pan gene symbol?: hsdM2
  • synonym:
  • product: type I restriction-modification system subunit M

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001802
  • symbol: JSNZ_001802
  • product: type I restriction-modification system subunit M
  • replicon: chromosome
  • strand: -
  • coordinates: 1849597..1851153
  • length: 1557
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCG
    ATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTAGGC
    TTGATTTTCTATCGCTTCTTATCTGAAAAAGCTGAACAAGAATATGCAGATGCCTTGGCA
    GGTGAAGATATTACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGATTTAAAA
    GCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGCGCGATG
    ATTCGTGAAATTGAAACGCAAGATTTCGATATCGAACACCTGACGACGGCAATTCGTAAA
    GTTGAAACATCAACACTAGGTGAAGAAAGTGAAAATGACTTTATCGGACTGTTCAGCGAT
    ATGGATTTGAGTTCAACACGTTTAGGTAACAATGTCAAAGAACGTACTGCGTTAATTTCC
    AAAGTTATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGATATG
    TTAGGTGATGCATACGAATTCCTTATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCT
    GGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGT
    AAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCATTGTTGTTACGT
    GTAGGTAAAGAGACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACATAC
    AACTTAGCACGGATGAATATGTTATTACATGATGTGCGTTATGAAAATTTTGATATCCGT
    AATGATGACACGTTGGAAAATCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCG
    AACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGATGAACGCTTC
    AGCGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACACATG
    GTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTATTC
    CGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAA
    GCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATCTTA
    GTATTTAAAAAATGCCGCCAACAAGACGACAACGTATTATTTATCGATGCATCCAATGAT
    TTTGAAAAAGGAAAAAATCAAAACCATTTAAGCGATGCCCAAGTCGAACGTATTATAGAC
    ACATATAAGCGTAAAGCAACGATTGATAAATATAGCTACAGCGCGACATTACAAGAGATT
    GCCGATAACGATTACAACTTAAACATACCGAGATATGTCGATACATTCGAAGAAGAAGCG
    CCAATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGACAAAGAAATCGCAGAA
    ATTGAACAAGAAATCAATGCATACCTGAAAGAACTAGGGGTGTTGAAAGATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001802
  • symbol: JSNZ_001802
  • description: type I restriction-modification system subunit M
  • length: 518
  • theoretical pI: 4.3956
  • theoretical MW: 59399.2
  • GRAVY: -0.481081

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.2)
    and 5 more
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 37.5)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.3)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.5)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 13.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 377.2)
    and 10 more
    MmeI_HD-like (CL0884) HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 68.5)
    NADP_Rossmann (CL0063) Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 37.3)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 25.5)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
    AviRa; RRNA methyltransferase AviRa (PF11599; HMM-score: 15.6)
    UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 14.4)
    no clan defined Mu_Gam-Sipho_Gp157-TOTE; Mu Gam/Sipho_Gp157-like domain of the TOTE conflict systems (PF22709; HMM-score: 12.4)
    Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 11.2)
    RBG (CL0760) BLTP3A_B; Bridge-like lipid transfer protein family member 3A/B (PF24917; HMM-score: 10.5)
    no clan defined CR6_interact; Growth arrest and DNA-damage-inducible proteins-interacting protein 1 (PF10147; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9916
    • Cytoplasmic Membrane Score: 0.0032
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0048
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00283
    • TAT(Tat/SPI): 0.000514
    • LIPO(Sec/SPII): 0.000637
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALAGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLTTAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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