PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1752 [new locus tag: SAUSA300_RS09585 ]
• pan locus tag^?: SAUPAN004556000
• symbol: hsdM
• pan gene symbol^?: hsdM2
• synonym:
• product: type I restriction-modification system, M subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1752 [new locus tag: SAUSA300_RS09585 ]
• symbol: hsdM
• product: type I restriction-modification system, M subunit
• replicon: chromosome
• strand: -
• coordinates: 1936836..1938575
• length: 1740
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913010 NCBI
• RefSeq: YP_494443 NCBI
• BioCyc: see SAUSA300_RS09585
• MicrobesOnline: 1293267 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGC
  GCCACTAAAACTCAAAATTTCCACCACCAACATCCAAATTATCAACATCGCAACATAACC
  AAATGTTATAATAAATCTATTACACAAAGAGATAAATTACTTATGCAAAGGCGGAGGAAT
  CACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
  TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTA
  GGCTTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTG
  TCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGACTTA
  AAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
  ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGC
  AAAGTTGAAACATCTACATTAGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGC
  GATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCTTTAATC
  TCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
  ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAA
  GCAGGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGAC
  GGTAAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCACTGTTGTTA
  CGTGTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACA
  TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATC
  CGTAATGATGACACATTGGAAAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATT
  GCGAACCCACCGTATAGTGCGAAATGGACTGCAGATTCAAAGTTTGAAAATGACGAACGA
  TTCAGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACAC
  ATGGTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTA
  TTCCGAGGTGCTGCAGAAGGTGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTA
  GAAGCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATT
  TTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
  GATTTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATA
  GACACATATAAGCGTAAGGAAACAATTGATAAATATAGCTACAGCGCGACACTACAAGAG
  ATTGCCGATAACGATTACAACCTAAATATACCGAGATATGTCGATACATTCGAAGAAGAA
  GCACCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCA
  GAAATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1752 [new locus tag: SAUSA300_RS09585 ]
• symbol: HsdM
• description: type I restriction-modification system, M subunit
• length: 579
• theoretical pI: 4.77081
• theoretical MW: 66947.7
• GRAVY: -0.562522

⊟Function

• reaction:
  EC 2.1.1.72?  ExPASy
  Site-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
• TIGRFAM:
  Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 467.2)
  and 5 more
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 36.5)
  Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 36.4)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 14.2)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 13.8)
• TheSEED  :
  • Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  and 1 more
  DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
• PFAM:
  NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 372)
  and 7 more
  no clan defined HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 75)
  NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 24.2)
  Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 23.7)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 15.7)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 14.2)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.1)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 13.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.060796
  • TAT(Tat/SPI): 0.001007
  • LIPO(Sec/SPII): 0.013465
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161919 NCBI
• RefSeq: YP_494443 NCBI
• UniProt: A0A0H2XJM2 UniProt

⊟Protein sequence

• MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hsdS < hsdM

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications