PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1751 [new locus tag: SAUSA300_RS09580 ]
• pan locus tag^?: SAUPAN004550000
• symbol: hsdS
• pan gene symbol^?: hsdS2
• synonym:
• product: type I restriction-modification enzyme, S subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1751 [new locus tag: SAUSA300_RS09580 ]
• symbol: hsdS
• product: type I restriction-modification enzyme, S subunit
• replicon: chromosome
• strand: -
• coordinates: 1935644..1936843
• length: 1200
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914124 NCBI
• RefSeq: YP_494442 NCBI
• BioCyc: see SAUSA300_RS09580
• MicrobesOnline: 1293266 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAGTAATACACAAAAGAAAAATGTGCCAGAATTGAGATTCCCAGGATTTGAAGGCGAA
  TGGGAAGAGAAGAAGTTAGGGAATCTTACTACCAAAATAGGTAGTGGAAAGACTCCCAAA
  GGTGGAAGTGAAAACTATACAAACAAAGGCATACCATTTTTAAGGAGTCAAAATATTAGA
  AATGGTAAATTAAATCTTAATGACTTAGTTTATATTAGTAAAGATATAGATGATGAGATG
  AAAAATAGTAGAACGTACTATGGTGATGTTCTTTTAAATATTACAGGAGCATCAATAGGT
  AGAACAGCCATTAATTCGATAGTTGAAACGCATGCTAATTTAAATCAACATGTATGTATT
  ATTAGATTGAAAAAAGAGTATTATTATAATTTTTTTGGACAGTATCTATTATCAAGAAAA
  GGTAAAAGGAAAATTTTCCTTGCACAAAGTGGAGGTAGTCGAGAAGGTCTAAACTTCAAA
  GAAATTGCTAATTTAAAAATCTTCACCCCAACTATATTTGAAGAACAGCAAAAAATAGGC
  AAGTTCTTCAGCAAACTCGACCGACAAATTGAATTAGAAGAACAAAAACTTGAATTGCTT
  CAACAACAGAAAAAAGGCTATATGCAGAAAATCTTCACACAAGAATTGCGATTCAAAGAT
  GAGAATGGTGAAGAATATCCAGAGTGGGAAAACAAATTCATAAAAGACATCTTTATCTTT
  GAAAATAATAGAAGAAAACCAATTACTTCTTCATTAAGAGAAAAGGGGTTATACCCTTAC
  TATGGTGCAACTGGAATTATTGATTACGTAAAAGATTATTTATTCAATAATGAAGAACGA
  TTACTAATAGGAGAAGATGGTGCAAAATGGGGGCAGTTTGAGACGAGTAGCTTTATTGCT
  AATGGGCAATACTGGGTAAATAATCATGCGCATGTAGTTAAAAGTAATGATCATAATTTG
  TTTTTTATGAATTATTATTTAAATTTTAAAGAACTACGAGCATTTGTCACAGGTAATGCA
  CCAGCTAAATTAACTCATGCGAACTTATGCAATATAAATCTTAAAATACCTTGTCTCACT
  GAACAAGATAAAGTAAGTGCATTGTTAAAATCTATAGACAATAAAATGAATAATCAAATG
  AATAGAATTGAGTTATTAAAAGAACGTAAAAAAGGACTATTACAAAAAATGTTTATTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1751 [new locus tag: SAUSA300_RS09580 ]
• symbol: HsdS
• description: type I restriction-modification enzyme, S subunit
• length: 399
• theoretical pI: 9.81387
• theoretical MW: 46467.8
• GRAVY: -0.66015

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 13.4)
• TheSEED  :
  • Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
  DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
  and 1 more
  DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
• PFAM:
  TRD (CL0477) Methylase_S; Type I restriction modification DNA specificity domain (PF01420; HMM-score: 143.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.049488
  • TAT(Tat/SPI): 0.002377
  • LIPO(Sec/SPII): 0.003703
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161451 NCBI
• RefSeq: YP_494442 NCBI
• UniProt: A0A0H2XIK8 UniProt

⊟Protein sequence

• MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hsdS < hsdM

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications