PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1625 [new locus tag: SA_RS09145 ]
• pan locus tag^?: SAUPAN004550000
• symbol: SA1625
• pan gene symbol^?: hsdS2
• synonym:
• product: specificity determinant HsdS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1625 [new locus tag: SA_RS09145 ]
• symbol: SA1625
• product: specificity determinant HsdS
• replicon: chromosome
• strand: -
• coordinates: 1857930..1859159
• length: 1230
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124468 NCBI
• RefSeq: NP_374914 NCBI
• BioCyc: see SA_RS09145
• MicrobesOnline: 103940 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAGTAATACACAAACGAAAAATGTGCCAGAGTTGAGATTCCCAGGATTTGAGGGCGAA
  TGGGAAGAGAAGAAGTTAGGGAATCTTACTACCAAAATAGGTAGTGGAAAGACTCCCAAA
  GGTGGAAGTGAAAACTATACAAACAAAGGCATACCATTTTTAAGGAGTCAAAATATTAGA
  AATGGTAAATTAAATCTTAATGACTTAGTTTATATTAGTAAAGATATAGATGATGAGATG
  AAAAATAGTAGAACGTACTATGGTGATGTTCTTTTAAATATTACAGGAGCATCAATAGGT
  AGAACAGCCATTAATTCGATAGTTGAAACGCATGCTAATTTAAATCAACATGTATGTATT
  ATTAGATTAAAAAAAGAGTATTATTATAATTTTTTTGGACAGTATCTATTATCAAGAAAA
  GGTAAAAGAAAAATTTTCCTTGCACAAAGTGGAGGTAGTCGAGAAGGACTAAACTTCAAA
  GAAATTGCTAATTTAAAAATCTTCACCCCAACTATATTTGAAGAACAACAAAAAATAGGA
  CAATTCTTCAGCAAACTTGACCAACAAATTGAATTAGAAGAACAAAAACTTGAATTACTT
  CAACAACAGAAAAAATGCTATATACAGAAAATCTTCTCACAAGAATTACGATTCAAAGAT
  GAAGAAGGTAATTACTATAAAGGATGGAACAAAAAGCAATTAAAAGATGTATTAGAATTT
  AGTAATAAAAGAACTATTAATGAAAATGAATATCCTGTTTTAACATCGTCAAGACAAGGT
  TTAATACTTCAGTCAGACTACTATAAAGATAGGAAAACTTTTGCAGAGAGTAATATTGGG
  TATTTCATACTCCCTAAAAATCATATAACATACCGTTCAAGAAGCGACGATGGAATTTTT
  AAGTTTAATTTAAATCTAATGATTGATGTAGGTATTATTAGTAAATATTACCCTGTCTTT
  AAAGGGATAGATGCAAATCAATATTATTTAACATTACACTTAAACTATCAACTGAAAAAA
  GAATATATTAAATATGCAACTGGTACATCACAATTGGTACTCTCACAAAAAGACTTGCAA
  AACATAAAGACTAAATTGCCATCTTATGAAGAACAACAAAAAATCGGTGATTTTTTCAGT
  GAAATAGATAGATTGGTTGAAAAACAATCTTCAAAAGTCGGACGATTAAAAGTACGTAAA
  AAAGAACTATTACAAAAAATGTTTGTTTAA
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⊟Protein

⊟General

• locus tag: SA1625 [new locus tag: SA_RS09145 ]
• symbol: SA1625
• description: specificity determinant HsdS
• length: 409
• theoretical pI: 9.95669
• theoretical MW: 47802.3
• GRAVY: -0.693643

⊟Function

• TIGRFAM:
• TheSEED  :
  • Type I restriction-modification system, specificity subunit S
  DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
  and 1 more
  DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
• PFAM:
  TRD (CL0477) Methylase_S; Type I restriction modification DNA specificity domain (PF01420; HMM-score: 116.5)
  and 1 more
  no clan defined CitX; Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (PF03802; HMM-score: 18.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.043135
  • TAT(Tat/SPI): 0.002275
  • LIPO(Sec/SPII): 0.003105
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927381 NCBI
• RefSeq: NP_374914 NCBI
• UniProt: A0A0H3JLG8 UniProt

⊟Protein sequence

• MSNTQTKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQQKIGQFFSKLDQQIELEEQKLELLQQQKKCYIQKIFSQELRFKDEEGNYYKGWNKKQLKDVLEFSNKRTINENEYPVLTSSRQGLILQSDYYKDRKTFAESNIGYFILPKNHITYRSRSDDGIFKFNLNLMIDVGIISKYYPVFKGIDANQYYLTLHLNYQLKKEYIKYATGTSQLVLSQKDLQNIKTKLPSYEEQQKIGDFFSEIDRLVEKQSSKVGRLKVRKKELLQKMFV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1625 < SA1626

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications