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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0171 [new locus tag: NWMN_RS00945 ]
  • pan locus tag?: SAUPAN001096000
  • symbol: NWMN_0171
  • pan gene symbol?: fadX
  • synonym:
  • product: acetyl-CoA/acetoacetyl-CoA transferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0171 [new locus tag: NWMN_RS00945 ]
  • symbol: NWMN_0171
  • product: acetyl-CoA/acetoacetyl-CoA transferase
  • replicon: chromosome
  • strand: -
  • coordinates: 220737..222314
  • length: 1578
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
    GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
    GCTGTGTTAGCGCAACATGACACATATCATACGCCCAATGATTTAACGTTTATATTAGCG
    AATGATATTCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
    ATTAAACGTGTCATTATGAGTATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
    AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
    AGTAATCAATTATTACCTGGAGTTATTACTAAAATCGGATTAAACACAGCTGTTGATCCT
    AGATACGGTGGCGGTAAAGTAAATACACGAACAACGGATGATTTAGTTTCATTAGTAACC
    ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
    GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
    TGTTATCATGTCGCATTAAACGCGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
    GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
    GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAAAGTCATTAT
    TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
    AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
    AGCATCGGTTATGGCATCAATAATGAACTCTCTAATTTATTGCATGAAGAATGTGTTGAA
    CATGATGTGCAACCGATTTTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
    CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
    ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
    AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGTGGCTTTATAGACATT
    ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
    TGCCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
    GTGAGCCATATCGACTTTAATGCACAATATTCACAATCACTCGAGCAAGAAATCTATTTT
    GTTACTGATCGTGCAGTATTCGAACTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
    CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
    GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
    TCAATTCATAAAGTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0171 [new locus tag: NWMN_RS00945 ]
  • symbol: NWMN_0171
  • description: acetyl-CoA/acetoacetyl-CoA transferase
  • length: 525
  • theoretical pI: 5.49984
  • theoretical MW: 58876.2
  • GRAVY: -0.163238

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 39.3)
    3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmalonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
    MetabolismEnergy metabolismOthermalonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
  • TheSEED:  
    FIG01108629: hypothetical protein 
  • PFAM:
    ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 71.4)
    Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 22.5)
    AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 17.7)
    no clan definedCurlin_rpt; Curlin associated repeat (PF07012; HMM-score: 11.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.172
    • Ymax_pos: 45
    • Cmax: 0.133
    • Cmax_pos: 45
    • Smax: 0.412
    • Smax_pos: 39
    • Smean: 0.131
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]