From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0169 [new locus tag: NWMN_RS00935 ]
  • pan locus tag?: SAUPAN001094000
  • symbol: fadD
  • pan gene symbol?: fadD
  • synonym:
  • product: acyl-CoA dehydrogenase FadD-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0169 [new locus tag: NWMN_RS00935 ]
  • symbol: fadD
  • product: acyl-CoA dehydrogenase FadD-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 217883..219139
  • length: 1257
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCATTTCACATTAGTATTCATATTATTTTTAGGAGGAATTTATATGACATTTGAAAAA
    GAAACGGTCTTAAAAACATTATTTCCTGAAGATGTACTTAGTATTGCTAAAGGTTTAACA
    GACGGTGAAGTCGAATTTTTACAACAAGTAGATTCATTGCTAGAAAGTAAGTACCGTGAA
    AATATTAATCAACATTGGATAGACGCTACTGTACCCGAGGACTATTTTAAAGATCTGGGA
    GAATTAAATTATTTTAACAATCCATTACTTTACAAGGATCGTCCAAACGCCAAAATGCCT
    AGTCAACTATTTCAGTTTTTCATGTCTTACCTACTCGCGCGATTTGATATTTCCTTAGCT
    ACCCTACTCGGTGTTCACCAAGGTTTAGGGCATAACACTTTCTATTTCGGAGGTAGCAAA
    GAACAAATTGCGAAATATGTACCTAAATTACAGTCACATGAACTGCGTACATGCTTTGCT
    TTAACTGAACCAGAACACGGTTCGGACGTTGCGGGAGGTCTTGAAACAGTCGCTGAACGC
    CAAGGCGATACTTGGGTTATCAATGGTGAAAAGAAATGGATTGGTGGTGCACATGTATCT
    GATGTCATTCCAGTATTCGCAGTAAATAAAGAAACTGGCAAACCCCATTGCTTTGTAGTC
    AGACCAGAACAAGATGGCGTCGATATTGAAGTCATTGATAATAAAATCGCACTTCGCATT
    GTTCCTAACGCCCTAATTAAATTAACTAATGTCAAAGTAGATGAAGCGGATCGCTTACAA
    AACATAACAAGCTTTAAAGATATTGCCAAAATTCTTTATTCAACGAGAGCAGGCGTTGCT
    TATATGGCTACAGGTGGTATGGCTGGCGCTTTACGTGCCACATTAGATTATGTCACTGAG
    CGTAAGCAATTCGGCAAACCAATTAGTAAATATCAGTTAATACAAGAAAAGCTAGCAATG
    ATGCAAGGTAATTTAGCTCAAGCAATGGCAACATGTGCTCAATTAGCTAATATGCAAGCA
    CATGGTGAATATGACGAGGTTGCAACTTCAACGGCGAAGATGATGAATGCCTTACGTTTG
    CGTGAGACAGTAGCTATGGGCCGCGGTATTACAGGTGGTAATGGCATACTAGCTGACGAT
    TATGATATTGCACGTTTCTTCTCTGATGCAGAAGCGATTTACACGTACGAAGGTACACAT
    GAAATTAATGCCTTAGTAATTGGACGCGCTTTGACTGGAGATTCTGCTTTCGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1257

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0169 [new locus tag: NWMN_RS00935 ]
  • symbol: FadD
  • description: acyl-CoA dehydrogenase FadD-like protein
  • length: 418
  • theoretical pI: 5.30054
  • theoretical MW: 46481.9
  • GRAVY: -0.0937799

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    cyclohexanecarboxyl-CoA dehydrogenase (TIGR03207; EC 1.1.1.-; HMM-score: 72.8)
    pimeloyl-CoA dehydrogenase, small subunit (TIGR03203; EC 1.3.1.62; HMM-score: 59.3)
    pimeloyl-CoA dehydrogenase, large subunit (TIGR03204; EC 1.3.1.62; HMM-score: 55.4)
    MetabolismCentral intermediary metabolismSulfur metabolismsulfur acquisition oxidoreductase, SfnB family (TIGR04022; EC 1.-.-.-; HMM-score: 34.8)
  • TheSEED:  
    Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8) 
  • PFAM:
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_1; Acyl-CoA dehydrogenase, C-terminal domain (PF00441; HMM-score: 93.8)
    no clan definedAcyl-CoA_dh_M; Acyl-CoA dehydrogenase, middle domain (PF02770; HMM-score: 69.5)
    Acyl-CoA_dh (CL0087) Acyl-CoA_dh_2; Acyl-CoA dehydrogenase, C-terminal domain (PF08028; HMM-score: 31.3)
    AcylCoA_ox_dh_N (CL0544) Acyl-CoA_dh_N; Acyl-CoA dehydrogenase, N-terminal domain (PF02771; HMM-score: 30.5)
    no clan definedDUF3726; Protein of unknown function (DUF3726) (PF12525; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 20
    • Cmax: 0.124
    • Cmax_pos: 20
    • Smax: 0.22
    • Smax_pos: 1
    • Smean: 0.121
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHFTLVFILFLGGIYMTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYRENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDISLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLETVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVIDNKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGALRATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVATSTAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVIGRALTGDSAFV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]